LOCUS       Exported                4755 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pSRFPS1, complete sequence.
ACCESSION   MF769791
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Rodriguez MD, Paul Z, Wood CE, Rice KC, Triplett EW.
  TITLE     Construction of Stable Fluorescent Reporter Plasmids for Use in 
            Staphylococcus aureus
  JOURNAL   Front Microbiol 8, 2491 (2017)
  PUBMED    29312199
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Rodriguez MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2017) Microbiology and Cell Science, University of
            Florida, 1355 Museum Rd., PO Box 110700, Gainesville, FL 32611-0700,
            USA
REFERENCE   3  (bases 1 to 4755)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Reporter vector pSRFPS1"
                     /mol_type="other DNA"
                     /db_xref="taxon:2073110"
     CDS             1..996
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /label=repF
                     /protein_id="AUS76875.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            1..996
                     /gene="repF"
                     /label=repF
     rep_origin      1147..1570
                     /label=R6K origin
                     /note="R6K origin"
     rep_origin      1179..1567
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /regulatory_class="promoter"
                     /note="sarA P1 promoter"
     CDS             1847..2332
                     /codon_start=1
                     /gene="dfrA"
                     /product="DfrA"
                     /label=dfrA
                     /protein_id="AUS76876.1"
                     /translation="MTLSIIVAHDKQRVIGYQNQLPWHLPNDLKHIKQLTTGNTLVMAR
                     KTFNSIGKPLPNRRNVVLTNQASFRHEGVDVINSLDEIKELSGHVFIFGGQTLYEAMID
                     QVDDMYITVIDGKFQGDTFFPPYTFENWEVESSVEGQLDEKNTIPHTFLHLVRRKGK"
     gene            1847..2332
                     /gene="dfrA"
                     /label=dfrA
     CDS             2367..3044
                     /codon_start=1
                     /gene="dsred"
                     /product="far-red fluorescent protein eqFP650"
                     /label=dsred
                     /protein_id="AUS76877.1"
                     /translation="MDNTEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     gene            2367..3044
                     /gene="dsred"
                     /label=dsred
     regulatory      3481..3838
                     /regulatory_class="terminator"
                     /note="atl transcription terminator"
     rep_origin      3935..4112
                     /label=sso
                     /note="sso"
     misc_feature    4581..4601
                     /label=dso
                     /note="dso"