LOCUS       Exported                5936 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSRalphapuroG, complete sequence.
ACCESSION   AY613996
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     A family of mammalian expression vectors with different selection 
            markers
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard
            des Prairies, Laval, Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 5936)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Cloning vector pSRalphapuroG"
                     /mol_type="other DNA"
                     /db_xref="taxon:279782"
     misc_feature    100..686
                     /label=ECMV internal ribosomal entry site
                     /note="ECMV internal ribosomal entry site"
     misc_feature    100..673
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    786..1360
                     /label=ECMV internal ribosomal entry site
                     /note="ECMV internal ribosomal entry site"
     misc_feature    786..1359
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1381..2100
                     /codon_start=1
                     /gene="hrGFP"
                     /product="green fluorescent protein"
                     /label=hrGFP
                     /protein_id="AAT48425.1"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     gene            1381..2100
                     /gene="hrGFP"
                     /label=hrGFP
     misc_feature    2143..2281
                     /label=contains SV40 polyA signal
                     /note="contains SV40 polyA signal"
     polyA_signal    2144..2278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2349..2948)
                     /codon_start=1
                     /gene="pac"
                     /product="puromycin N-acetyltransferase"
                     /label=pac
                     /protein_id="AAT48426.1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTLDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     gene            complement(2349..2948)
                     /gene="pac"
                     /label=pac
     promoter        complement(2958..3287)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      complement(2968..3296)
                     /regulatory_class="promoter"
                     /note="SV40 early"
     rep_origin      2972..3107
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3322..3426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3427..4287
                     /codon_start=1
                     /gene="bla"
                     /product="ampicillin resistance protein"
                     /label=bla
                     /protein_id="AAT48427.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3427..4287
                     /gene="bla"
                     /label=bla
     rep_origin      4410..5269
                     /label=ColE1
                     /note="ColE1"
     rep_origin      4458..5046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      5299..5926
                     /regulatory_class="promoter"
                     /note="SRalpha"
     promoter        5308..5921
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      5488..5623
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             5653..5921
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"