LOCUS       Exported                6588 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSRalphahygroY, complete sequence.
ACCESSION   AY613998
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     A family of mammalian expression vectors with different selection 
            markers
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard
            des Prairies, Laval, Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 6588)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Cloning vector pSRalphahygroY"
                     /mol_type="other DNA"
                     /db_xref="taxon:279784"
     misc_feature    100..686
                     /label=ECMV internal ribosomal entry site
                     /note="ECMV internal ribosomal entry site"
     misc_feature    100..673
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    786..1360
                     /label=ECMV internal ribosomal entry site
                     /note="ECMV internal ribosomal entry site"
     misc_feature    786..1359
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      1425..1434
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1431..2150
                     /codon_start=1
                     /gene="YFP"
                     /product="yellow fluorescent protein"
                     /label=YFP
                     /protein_id="AAT48431.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            1431..2150
                     /gene="YFP"
                     /label=YFP
     polyA_signal    2173..2307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2180..2307
                     /label=contains SV40 polyA signal
                     /note="contains SV40 polyA signal"
     polyA_signal    2343..2391
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(2433..3470)
                     /codon_start=1
                     /gene="hph"
                     /product="hygromycin B phosphotransferase"
                     /label=hph
                     /protein_id="AAT48432.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRGDRE
                     MGEAN"
     gene            complement(2433..3470)
                     /gene="hph"
                     /label=hph
     promoter        complement(3610..3939)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      complement(3620..3948)
                     /regulatory_class="promoter"
                     /note="SV40 early"
     rep_origin      3624..3759
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3974..4078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4079..4939
                     /codon_start=1
                     /gene="bla"
                     /product="ampicillin resistance protein"
                     /label=bla
                     /protein_id="AAT48433.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4079..4939
                     /gene="bla"
                     /label=bla
     rep_origin      5110..5698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5126..5700
                     /label=ColE1
                     /note="ColE1"
     regulatory      5951..6573
                     /regulatory_class="promoter"
                     /note="SRalpha"
     promoter        5960..6573
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      6140..6275
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             6305..6573
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"