LOCUS       Exported                5800 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSRalphablastC, complete sequence.
ACCESSION   AY613995
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     A family of mammalian expression vectors with different selection 
            markers
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Allard V, de Leseleuc L, Denis F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2004) INRS-Institut Armand-Frappier, 531 Boulevard
            des Prairies, Laval, Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 5800)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Cloning vector pSRalphablastC"
                     /mol_type="other DNA"
                     /db_xref="taxon:279781"
     misc_feature    1..252
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    100..686
                     /label=ECMV internal ribosomal entry site
                     /note="ECMV internal ribosomal entry site"
     misc_feature    100..673
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    786..1360
                     /label=ECMV internal ribosomal entry site
                     /note="ECMV internal ribosomal entry site"
     misc_feature    786..1359
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      1425..1434
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1431..2150
                     /codon_start=1
                     /gene="CFP"
                     /product="cyan fluorescent protein"
                     /label=CFP
                     /protein_id="AAT48422.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            1431..2150
                     /gene="CFP"
                     /label=CFP
     misc_feature    2172..2310
                     /label=contains SV40 polyA signal
                     /note="contains SV40 polyA signal"
     polyA_signal    2173..2307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2313..2735)
                     /codon_start=1
                     /gene="bsr"
                     /product="blasticidin S deaminase"
                     /label=bsr
                     /protein_id="AAT48423.1"
                     /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS
                     AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR
                     ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     gene            complement(2313..2735)
                     /gene="bsr"
                     /label=bsr
     promoter        complement(2745..2763)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2755..2802)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2822..3151)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      complement(2832..3167)
                     /regulatory_class="promoter"
                     /note="SV40 early"
     rep_origin      2836..2971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3186..3290
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3291..4151
                     /codon_start=1
                     /gene="bla"
                     /product="ampicillin resistance protein"
                     /label=bla
                     /protein_id="AAT48424.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3291..4151
                     /gene="bla"
                     /label=bla
     rep_origin      4322..4910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      4338..4912
                     /label=ColE1
                     /note="ColE1"
     regulatory      5163..5785
                     /regulatory_class="promoter"
                     /note="SRalpha"
     promoter        5172..5785
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      5352..5487
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             5517..5785
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"