LOCUS       Exported                3624 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSR26-2, complete sequence.
ACCESSION   MG323877
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Landry BP, Palanki R, Dyulgyarov N, Hartsough LA, Tabor JJ.
  TITLE     Phosphatase activity tunes two-component system sensor detection 
            threshold
  JOURNAL   Nat Commun 9 (1), 1433 (2018)
  PUBMED    29650958
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Landry BP.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2017) Bioengineering, Rice University, 6100 Main 
            St, Houston, TX 77005, USA
REFERENCE   3  (bases 1 to 3624)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Cloning vector pSR26-2"
                     /mol_type="other DNA"
                     /db_xref="taxon:2219088"
     misc_feature    complement(1..6)
                     /label=AvrII Scar
                     /note="AvrII Scar"
     misc_feature    complement(6..819)
                     /label=ColE1 Fragment
                     /note="ColE1 Fragment"
     rep_origin      complement(133..815)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
     rep_origin      complement(151..739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(151..703)
                     /label=RNA II
                     /note="RNA II"
     regulatory      557..562
                     /regulatory_class="minus_35_signal"
     regulatory      580..586
                     /regulatory_class="minus_10_signal"
     misc_feature    593..700
                     /label=RNAI
                     /note="RNAI"
     regulatory      complement(711..717)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(735..740)
                     /regulatory_class="minus_35_signal"
     misc_feature    complement(763..815)
                     /label=Tn3 Artifact
                     /note="Tn3 Artifact"
     misc_feature    complement(815..820)
                     /label=SpeI Scar
                     /note="SpeI Scar"
     regulatory      complement(820..925)
                     /regulatory_class="terminator"
                     /note="T0"
     terminator      827..921
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    complement(927..1721)
                     /label=CamR Cassette from E. coli R6-5 plasmid
                     /note="CamR Cassette from E. coli R6-5 plasmid"
     misc_feature    complement(927..932)
                     /label=SacI Scar
                     /note="SacI Scar"
     CDS             complement(942..1601)
                     /codon_start=1
                     /product="CmR"
                     /label=CmR
                     /protein_id="AWU50977.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1602..1704)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     misc_feature    1751..1801
                     /label=BW25113 LacZ Upstream
                     /note="BW25113 LacZ Upstream"
     misc_feature    1803..1808
                     /label=XhoI Scar
                     /note="XhoI Scar"
     promoter        1809..1882
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     regulatory      1809..1862
                     /regulatory_class="promoter"
                     /note="PLTetO-1"
     misc_feature    1809..1827
                     /label=TetR1
                     /note="TetR1"
     protein_bind    1809..1827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      1828..1833
                     /regulatory_class="minus_35_signal"
     misc_feature    1834..1852
                     /label=TetR2
                     /note="TetR2"
     protein_bind    1834..1852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      1851..1856
                     /regulatory_class="minus_10_signal"
     misc_feature    1863..1882
                     /label=Lambda Phage Fragment
                     /note="Lambda Phage Fragment"
     misc_feature    1863
                     /label=+1
                     /note="+1"
     regulatory      1883..1917
                     /regulatory_class="ribosome_binding_site"
                     /note="sRBS (500)"
     CDS             1918..2637
                     /codon_start=1
                     /product="OmpR"
                     /label=OmpR
                     /note="ompR"
                     /protein_id="AWU50975.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLLT
                     RESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDYIP
                     KPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMPLTS
                     GEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAHPRYI
                     QTVWGLGYVFVPDGSKA"
     misc_feature    1936..2265
                     /label=Phosphoacceptor domain
                     /note="Phosphoacceptor domain"
     misc_feature    2284..2328
                     /label=Linker
                     /note="Linker"
     misc_feature    2386..2616
                     /label=DNA-binding domain
                     /note="DNA-binding domain"
     regulatory      2650..2778
                     /regulatory_class="terminator"
                     /note="B0015"
     regulatory      2650..2729
                     /regulatory_class="terminator"
                     /note="B0010"
     terminator      2658..2729
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    2730..2737
                     /label=BB Scar
                     /note="BB Scar"
     regulatory      2738..2778
                     /regulatory_class="terminator"
                     /note="B0012"
     terminator      2745..2772
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    2779..2823
                     /label=pUC MCS
                     /note="pUC MCS"
     regulatory      2824..2858
                     /regulatory_class="promoter"
                     /note="J23106"
     regulatory      2859..2892
                     /regulatory_class="ribosome_binding_site"
                     /note="sRBS (50,000)"
     CDS             2893..3519
                     /codon_start=1
                     /product="TetR"
                     /label=TetR
                     /note="tetR"
                     /protein_id="AWU50976.1"
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     regulatory      3520..3624
                     /regulatory_class="terminator"
                     /note="T1"
     terminator      3525..3611
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"