LOCUS       Exported                3457 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Poxvirus recombinant transfer vector pSPV-EGFP, complete sequence.
ACCESSION   GU062789
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Luo S, Peng Y, Diel DG, Rock DL.
  TITLE     Virus recombinant transfer vector pSPV-EGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Luo S, Peng Y, Diel DG, Rock DL.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2009) Pathobiology, University of Illinois at 
            Urbana-Champaign, 2001 S. Lincoln Avenue, Urbana, IL 61802, USA
REFERENCE   3  (bases 1 to 3457)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Poxvirus recombinant transfer vector pSPV-EGFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:685566"
     misc_feature    4..33
                     /label=multiple cloning site 1 (MCS)
                     /note="multiple cloning site 1 (MCS)"
     regulatory      52..320
                     /regulatory_class="promoter"
                     /note="early-late Vaccinia virus (VACV) VV7.5 promoter"
     regulatory      334..343
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             340..1059
                     /codon_start=1
                     /gene="egfp"
                     /product="enhanced green fluorescent protein"
                     /label=egfp
                     /note="EGFP; reporter"
                     /protein_id="ACY06902.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            340..1059
                     /gene="egfp"
                     /label=egfp
     misc_feature    1060..1085
                     /label=multiple cloning site 2 (MCS2)
                     /note="multiple cloning site 2 (MCS2)"
     regulatory      complement(1094..1113)
                     /regulatory_class="promoter"
                     /note="T7"
     promoter        complement(1095..1113)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1371..1959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2130..2990)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="BLA"
                     /protein_id="ACY06903.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2130..2990)
                     /gene="bla"
                     /label=bla
     promoter        complement(2991..3095)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3441..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      3441..3457
                     /regulatory_class="promoter"
                     /note="SP6 promoter"