LOCUS       Exported                4570 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pSpcP-lac, complete sequence.
ACCESSION   AF519494
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Chen Z, Zhang J, Huang D.
  TITLE     Escherichia coli-Pseudomonas shuttle plasmid pSpcP-lac based 
            spectinomycin resistant gene and lacZ alpha gene
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Chen Z, Zhang J, Huang D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2002) Biotechnology, Institute of Plant 
            Protection, 2, Yuan Ming Yuan Xi Lu, HaiDian District, Beijing, 
            Beijing 100094, China
REFERENCE   3  (bases 1 to 4570)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Shuttle vector pSpcP-lac"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:211784"
     CDS             1128..1910
                     /codon_start=1
                     /product="spectinomycin 3-adenylyltransferase"
                     /label=spectinomycin 3-adenylyltransferase
                     /protein_id="AAQ08404.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRIN
                     SDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYPPK
                     REFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDIR
                     RAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTLL
                     DIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     CDS             complement(3897..4259)
                     /codon_start=1
                     /product="LacZ alpha peptide"
                     /label=LacZ alpha peptide
                     /note="derived from from pBlueScript KS(-)"
                     /protein_id="AAQ08405.1"
                     /translation="MTMITPSSKLTLTKGNKSWVPGPPSRSTVSISLISNSCSPGDPLV
                     LERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWKL"
     primer_bind     4046..4062
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4069..4087
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4096..4203
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     4120..4136
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4170..4186)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4216..4234)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4255..4271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4279..4295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4303..4333)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4348..4369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."