LOCUS       Exported                3369 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSpark V, complete sequence.
ACCESSION   GQ489188
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Paz E.
  TITLE     pSpark V DNA Cloning Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Paz E.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2009) Molecular Biology, Canvax Biotech S.L., 
            Estocolmo, Cordoba, Cordoba 14014, Spain
REFERENCE   3  (bases 1 to 3369)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Cloning vector pSpark V"
                     /mol_type="other DNA"
                     /db_xref="taxon:673750"
     CDS             109..300
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      complement(732..1320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1491..2351)
                     /codon_start=1
                     /product="beta lactamase"
                     /label=beta lactamase
                     /protein_id="ACV85765.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2352..2456)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2534..2989)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3130..3146
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3153..3171
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3307..3325)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3343..3359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"