LOCUS       Exported                3634 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Microarray spiking control vector pSP64bb2sp3, complete sequence.
ACCESSION   DQ480367
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Sato M, Mitra RM, Coller J, Wang D, Spivey NW, Dewdney J, Denoux C, 
            Glazebrook J, Katagiri F.
  TITLE     A high-performance, small-scale microarray for expression profiling 
            of many samples in Arabidopsis-pathogen studies
  JOURNAL   Plant J. 49 (3), 565-577 (2007)
  PUBMED    17181774
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Sato M, Katagiri F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2006) Department of Plant Biology, University of 
            Minnesota, 1500 Gortner Avenue, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 3634)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Microarray spiking control vector pSP64bb2sp3"
                     /mol_type="other DNA"
                     /db_xref="taxon:382154"
     misc_feature    1..690
                     /label=transcribed region
                     /note="transcribed region"
     misc_feature    1..579
                     /label=backbone sequence 2 (bb2)
                     /note="backbone sequence 2 (bb2)"
     misc_feature    580..649
                     /label=spiking control 3 (sp3)
                     /note="spiking control 3 (sp3)"
     misc_feature    650..12
                     /label=pSP64 poly(A)-derived sequence
                     /note="pSP64 poly(A)-derived sequence"
     primer_bind     complement(704..720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    728..744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(752..782)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    797..818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1106..1694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1865..2725)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ABF47281.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1865..2725)
                     /gene="bla"
                     /label=bla
     promoter        complement(2726..2830)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3618..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"