LOCUS       Exported               11259 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSOL9LHGRP, complete sequence.
ACCESSION   JX185748
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Gase K, Meldau S, Schaefer M, Baldwin IT.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2012) Molecular Ecology, Max-Planck-Institute for 
            Chemical Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, 
            Germany
REFERENCE   2  (bases 1 to 11259)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Cloning vector pSOL9LHGRP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1307969"
     misc_feature    244..268
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    244..266
                     /label=left border T-DNA
                     /note="left border T-DNA"
     regulatory      complement(335..612)
                     /regulatory_class="terminator"
                     /note="NOS"
     terminator      342..594
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(616..1641)
                     /codon_start=1
                     /product="HptII"
                     /label=HptII
                     /note="hygromycin phosphotransferase II"
                     /protein_id="AGI38103.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     regulatory      complement(1642..1960)
                     /regulatory_class="promoter"
                     /note="NOS"
     promoter        complement(1662..1845)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(1698..1877)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      1967..2496
                     /regulatory_class="promoter"
                     /note="cauliflower mosaic virus"
     promoter        2155..2499
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2510..4735
                     /codon_start=1
                     /gene="lhgr-N"
                     /product="LhGR-N"
                     /label=lhgr-N
                     /note="fusion of the steroid binding domain of a rat 
                     glucocrticoid receptor (2516..3388), lacI-His17 
                     (3392..4378) and yeast GAL4 
                     transcription-activation-domain-II (4391..4735)"
                     /protein_id="AGI38105.1"
                     /translation="MASEARKTKKKIKGIQQATAGVSQDTSENPNKTIVPAALPQLTPT
                     LVSLLEVIEPEVLYAGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLD
                     DQMTLLQYSWMFLMAFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFV
                     SSELQRLQVSYEEYLCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNS
                     SQNWQRFYQLTKLLDSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNG
                     NIKKLLFHQKSTSKPVTLYDVAEYAGVSHQTVSRVVNQASHVSAKTREKVEAAMAELNY
                     IPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEA
                     CKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHED
                     GTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMS
                     GFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYI
                     PPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTSGSEFANFNQSGN
                     IADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSK
                     PLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE"
     gene            2510..4735
                     /gene="lhgr-N"
                     /label=lhgr-N
                     /note="synthetic gene from: Samalova, M. et al. (2005) 
                     pOp6/LhGR: A stringently regulated and highly responsive 
                     dexamethasone-inducible gene expression system for tobacco.
                     The Plant Journal 41: 919-935"
     CDS             4391..4735
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     regulatory      4995..5186
                     /regulatory_class="terminator"
                     /note="cauliflower mosaic virus"
     polyA_signal    5000..5174
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5245..5270
                     /label=right border T-DNA
                     /note="right border T-DNA"
     misc_feature    5246..5270
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5379..6276
                     /label=ColE1 type from pUC19
                     /note="ColE1 type from pUC19"
     rep_origin      5627..6215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      6658..6852
                     /label=pVS1
                     /note="pVS1"
     CDS             complement(6918..8093)
                     /codon_start=1
                     /product="pVS1 RepA"
                     /label=pVS1 RepA
                     /note="pVS1 replication protein"
                     /protein_id="AGI38101.1"
                     /translation="MPHVWRNGRLARRKRLGCLPALQWHWNPQARGIGVSGRKPSGPVQ
                     IGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKP
                     PKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGP
                     AGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKD
                     DLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELAD
                     WMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSA
                     GEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS"
     CDS             complement(6918..7991)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8420..9049)
                     /codon_start=1
                     /product="pVS1 StaA"
                     /label=pVS1 StaA
                     /note="pVS1 partitioning protein"
                     /protein_id="AGI38102.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10459..11253
                     /codon_start=1
                     /product="NptII"
                     /label=NptII
                     /note="neomycin phosphotransferase II"
                     /protein_id="AGI38104.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"