LOCUS       Exported               11253 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSOL9LHGRC, complete sequence.
ACCESSION   JX185747
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Schafer M, Brutting C, Gase K, Reichelt M, Baldwin I, Meldau S.
  TITLE     Real time genetic manipulation: a new tool for ecological field 
            studies
  JOURNAL   Plant J. 76 (3), 506-518 (2013)
  PUBMED    23906159
REFERENCE   2  (bases 1 to 11253)
  AUTHORS   Gase K, Meldau S, Schaefer M, Baldwin IT.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2012) Molecular Ecology, Max-Planck-Institute for 
            Chemical Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, 
            Germany
REFERENCE   3  (bases 1 to 11253)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Cloning vector pSOL9LHGRC"
                     /mol_type="other DNA"
                     /db_xref="taxon:1307968"
     misc_feature    244..268
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    244..266
                     /label=left border T-DNA
                     /note="left border T-DNA"
     regulatory      complement(335..612)
                     /regulatory_class="terminator"
                     /note="NOS"
     terminator      342..594
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(616..1641)
                     /codon_start=1
                     /product="HptII"
                     /label=HptII
                     /note="hygromycin phosphotransferase II"
                     /protein_id="AGI38098.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     regulatory      complement(1642..1960)
                     /regulatory_class="promoter"
                     /note="NOS"
     promoter        complement(1662..1845)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(1698..1877)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      1967..2496
                     /regulatory_class="promoter"
                     /note="cauliflower mosaic virus"
     promoter        2155..2499
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2510..4729
                     /codon_start=1
                     /gene="lhgr-I"
                     /product="LhGR-I"
                     /label=lhgr-I
                     /note="fusion of lacI-His17 (2513..3499), the steroid 
                     binding domain of a rat glucocrticoid receptor (3503..4369)
                     and yeast GAL4 transcription-activation-domain-II 
                     (4379..4729)"
                     /protein_id="AGI38100.1"
                     /translation="MKPVTLYDVAEYAGVSHQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTSEARKTKKKIK
                     GIQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSAWR
                     IMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSS
                     GNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEYLCMKTLLLLSSVP
                     KEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLTY
                     CFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQKSTSGSEFANFNQSGNIA
                     DSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPL
                     SPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE"
     gene            2510..4729
                     /gene="lhgr-I"
                     /label=lhgr-I
                     /note="synthetic gene from: Samalova, M. et al. (2005) 
                     pOp6/LhGR: A stringently regulated and highly responsive 
                     dexamethasone-inducible gene expression system for tobacco.
                     The Plant Journal 41: 919-935"
     CDS             4385..4729
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     regulatory      4989..5180
                     /regulatory_class="terminator"
                     /note="cauliflower mosaic virus"
     polyA_signal    4994..5168
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5239..5264
                     /label=right border T-DNA
                     /note="right border T-DNA"
     misc_feature    5240..5264
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5373..6270
                     /label=ColE1 type from pUC19
                     /note="ColE1 type from pUC19"
     rep_origin      5621..6209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      6652..6846
                     /label=pVS1
                     /note="pVS1"
     CDS             complement(6912..8087)
                     /codon_start=1
                     /product="pVS1 RepA"
                     /label=pVS1 RepA
                     /note="pVS1 replication protein"
                     /protein_id="AGI38096.1"
                     /translation="MPHVWRNGRLARRKRLGCLPALQWHWNPQARGIGVSGRKPSGPVQ
                     IGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKP
                     PKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGP
                     AGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKD
                     DLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELAD
                     WMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSA
                     GEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS"
     CDS             complement(6912..7985)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8414..9043)
                     /codon_start=1
                     /product="pVS1 StaA"
                     /label=pVS1 StaA
                     /note="pVS1 partitioning protein"
                     /protein_id="AGI38097.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10453..11247
                     /codon_start=1
                     /product="NptII"
                     /label=NptII
                     /note="neomycin phosphotransferase II"
                     /protein_id="AGI38099.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"