LOCUS       Exported                6888 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pSMT3-EO6, complete sequence.
ACCESSION   DQ115384
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice 
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2005) Medical Virology, University of Cape Town, 
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 6888)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Shuttle vector pSMT3-EO6"
                     /mol_type="other DNA"
                     /note="Mycobacterium-E. coli shuttle vector; murine EO 
                     rotavirus VP6 cloned into BamH1-Hind111 sites of pSMT3 
                     (D.B. Young)"
                     /db_xref="taxon:336702"
     regulatory      6..381
                     /regulatory_class="promoter"
                     /note="derived from Mycobacterium bovis hsp60 promoter of 
                     GenBank Accession Number M17705"
     CDS             381..1601
                     /codon_start=1
                     /product="Hsp60/VP6 chimera"
                     /label=Hsp60/VP6 chimera
                     /note="first six amino acids of Mycobacterium bovis hsp60 
                     protein linked via three amino acids to the 397 amino acid 
                     murine EO rotavirus VP6 protein"
                     /protein_id="AAZ16502.1"
                     /translation="MAKTIADPKMDVLYSISRTLKDARDKIVEGTLYSNVSDLIQQFNQ
                     MLVTMNGNEFQTGGIGNLPLRNWNFDFGLLGTTLLNLDANYVESARTTIDYFVDFIDNV
                     CMDEMVRESQRNGIAPQSDALRKLSGVKFRRINFNNSSEYIENWNLQNRRQRTGFTFHK
                     PNIFPYSASFTLNRSQPQHDNLMGTMWLNAGSEIQVAGFDYSCAINAPANIQQFEHIVQ
                     LRRVLTTATITLLPDAERFSFPRVINSADGATTWYFNPVILRPNNVEVEFLLNGQIINT
                     YQARFGTIVARNFDTIRLSFQLMRPPNMTPAVAALFPNAQPFEHHATVGLTLRIDSAIC
                     ESVLADASETMLANVTSVRQEYAIPVGPVFPPGMNWTDLITNYSPSREDNLQRVFTVAS
                     IRSMLVK"
     misc_feature    381..398
                     /label=derived from Mycobacterium bovis hsp60 protein
                     /note="derived from Mycobacterium bovis hsp60 protein"
     misc_feature    408..1601
                     /label=derived from murine EO rotavirus VP6
                     /note="derived from murine EO rotavirus VP6"
     primer_bind     complement(1613..1629)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1659..4246
                     /label=Mycobacterial OriM region derived from pAL5000
                     /note="Mycobacterial OriM region derived from pAL5000"
     misc_feature    4357..5603
                     /label=region of hygromycin resistance
                     /note="region of hygromycin resistance"
     rep_origin      5613..6851
                     /label=OriE region derived from pUC19
                     /note="OriE region derived from pUC19"
     rep_origin      5852..6440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6728..6749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6764..6794
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6802..6818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6826..6842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"