LOCUS       Exported                6900 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Large-insert cloning vector pSMART VC, complete sequence.
ACCESSION   AY643800
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Wild J, Hradecna Z, Szybalski W.
  TITLE     Conditionally amplifiable BACs: switching from single-copy to 
            high-copy vectors and genomic clones
  JOURNAL   Genome Res. 12 (9), 1434-1444 (2002)
  PUBMED    12213781
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Godiska R, Mead DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2004) Lucigen Corp., 2120 W. Greenview Dr. #9, 
            Middleton, WI 53562, USA
REFERENCE   3  (bases 1 to 6900)
  AUTHORS   Godiska R, Mead DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2005) Lucigen Corp., 2120 W. Greenview Dr. #9, 
            Middleton, WI 53562, USA
REFERENCE   4  (bases 1 to 6900)
  AUTHORS   Godiska R, Mead DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2005) Lucigen Corp., 2120 W. Greenview Dr. #9, 
            Middleton, WI 53562, USA
REFERENCE   5  (bases 1 to 6900)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Jun 27, 2005 this sequence version replaced AY643800.2.
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Large-insert cloning vector pSMART VC"
                     /mol_type="other DNA"
                     /db_xref="taxon:280595"
     terminator      28..57
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     protein_bind    79..112
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    176..231
                     /label=multiple cloning site
                     /note="multiple cloning site"
     protein_bind    273..306
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     terminator      325..356
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     misc_feature    384..813
                     /label=bacteriphage lambda cosN site
                     /note="bacteriphage lambda cosN site"
     misc_feature    405..803
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     promoter        825..927
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             928..1587
                     /codon_start=1
                     /gene="CAT"
                     /product="chloramphenicol acetyl transferase"
                     /label=CAT
                     /note="acetyl transferase"
                     /protein_id="AAT42373.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            928..1587
                     /gene="CAT"
                     /label=CAT
     misc_feature    1628..4548
                     /note="sequence derived from F plasmid in GenBank Accession
                     Number AP001918"
     repeat_region   1628..2330
                     /label=SOPC (IncD)
                     /note="SOPC (IncD)"
     misc_feature    1858..2331
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(2404..3375)
                     /codon_start=1
                     /gene="parB"
                     /product="plasmid partitioning protein SopB"
                     /label=parB
                     /protein_id="AAT42374.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(2404..3375)
                     /gene="parB"
                     /label=parB
     CDS             complement(3375..4550)
                     /codon_start=1
                     /gene="parA"
                     /product="plasmid partitioning protein SopA"
                     /label=parA
                     /protein_id="AAT42375.1"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(3375..4550)
                     /gene="parA"
                     /label=parA
     misc_feature    4876..5126
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    4878..6267
                     /note="sequence derived from F plasmid in GenBank Accession
                     Number AP001918"
     CDS             complement(5129..5884)
                     /codon_start=1
                     /gene="repE"
                     /product="RepE replication initiation protein"
                     /label=repE
                     /protein_id="AAT42376.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(5129..5884)
                     /gene="repE"
                     /label=repE
     rep_origin      5975..6194
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      5978..6267
                     /label=ori2 (oriS) single copy replication origin
                     /note="ori2 (oriS) single copy replication origin"
     rep_origin      6270..6884
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      6274..6900
                     /label=oriV inducible replication origin
                     /note="oriV inducible replication origin"