LOCUS       Exported                4801 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSM47, complete sequence.
ACCESSION   AY456754
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Fuchs U, Czymmek KJ, Sweigard JA.
  TITLE     Five hydrophobin genes in Fusarium verticillioides include two 
            required for microconidial chain formation
  JOURNAL   Fungal Genet. Biol. 41 (9), 852-864 (2004)
  PUBMED    15288021
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Sweigard J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2003) Crop Genetics, DuPont, PO 80402, Wilmington,
            DE 19880-0402, USA
REFERENCE   3  (bases 1 to 4801)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Cloning vector pSM47"
                     /mol_type="other DNA"
                     /db_xref="taxon:259680"
     misc_feature    195..306
                     /label=double loxP site
                     /note="double loxP site"
     protein_bind    complement(195..228)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(273..306)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        363..464
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             465..1139
                     /codon_start=1
                     /gene="TRP1"
                     /product="phosphoribosylanthranilate isomerase"
                     /label=TRP1
                     /protein_id="AAR87726.1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     gene            465..1139
                     /gene="TRP1"
                     /label=TRP1
     misc_feature    1194..2539
                     /note="derived from Saccharomyces cerevisiae 2 micron 
                     plasmid"
     rep_origin      1194..2536
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(2157..2204)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(2580..2596)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2604..2620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2628..2658)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2673..2694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2982..3570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3741..4601)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAR87725.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3741..4601)
                     /gene="bla"
                     /label=bla
     promoter        complement(4602..4706)
                     /gene="bla"
                     /label=AmpR promoter