LOCUS       Exported                5920 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSLIE1-3_Mos1mariner_ORF, complete sequence.
ACCESSION   JN707701
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Alrefaei YN, Suttiprapa S, Okatcha TI, Rinaldi G, Brindley PJ.
  TITLE     A plasmid encoding the Mos-1 mariner transposase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Alrefaei YN, Suttiprapa S, Okatcha TI, Rinaldi G, Brindley PJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2011) Microbiology, Immunology and Tropical 
            Medicine, George Washington University, 2300 Eye Street, NW, 
            Washington, DC 20037, USA
REFERENCE   3  (bases 1 to 5920)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Cloning vector pSLIE1-3_Mos1mariner_ORF"
                     /mol_type="other DNA"
                     /label=derived from Drosophila mauritiana
                     /note="derived from Drosophila mauritiana"
                     /db_xref="taxon:1140119"
     regulatory      1..488
                     /regulatory_class="enhancer"
                     /gene="hr5"
     gene            1..488
                     /gene="hr5"
                     /label=hr5
     enhancer        1..485
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        489..1080
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     regulatory      492..1087
                     /regulatory_class="promoter"
                     /gene="ie1"
     gene            492..1087
                     /gene="ie1"
                     /label=ie1
     CDS             1107..2144
                     /codon_start=1
                     /product="transposase"
                     /label=transposase
                     /protein_id="AEZ51500.1"
                     /translation="MSSFVPNKEQTRTVLIFCFHLKKTAAESHRMLVEAFGEQVPTVKT
                     CERWFQRFKSGDFDVDDKEHGKPPKRYEDAELQALLDEDDAQTQKQLAEQLEVSQQAVS
                     NRLREMGKIQKVGRWVPHELNERQMERRKNTCEILLSRYKRKSFLHRIVTGDEKWIFFV
                     NPKRKKSYVDPGQPATSTARPNRFGKKTMLCVWWDQSGVIYYELLKPGETVNTARYQQQ
                     LINLNRALQRKRPEYQKRQHRVIFLHDNAPSHTARAVRDTLETLNWEVLPHAAYSPDLA
                     PSDYHLFASMGHALAEQRFDSYESVKKWLDEWFAAKDDEFYWRGIHKLPERWEKCVASD
                     GKYFE"
     misc_feature    2157..3119
                     /label=contains polyA_site
                     /note="contains polyA_site"
     promoter        3735..3839
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3840..4700
                     /codon_start=1
                     /product="Amp"
                     /label=Amp
                     /protein_id="AEZ51501.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4871..5459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5458
     protein_bind    5747..5768
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5783..5813
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5821..5837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5845..5861
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"