LOCUS       Exported                7476 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSL720, complete sequence.
ACCESSION   KJ865751
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Hart BE, Asrican R, Lim S-Y., Chen C-J., Seay SA, Souther SJR., 
            Rayasam SDJ., Saelens JW, Larsen MH, Schmitz JE, Haynes BF, Jacobs 
            WR Jr., Lee S, Frothingham R.
  TITLE     Stable Expression of Lentiviral Antigens by Quality-Assured 
            Recombinant BCG Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Hart BE, Frothingham R, Lee S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2014) Duke Human Vaccine Institute, Duke 
            University, 911 S. Lasalle St., Durham, NC 27705, USA
REFERENCE   3  (bases 1 to 7476)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Cloning vector pSL720"
                     /strain="delta-leuCD Escherichia coli"
                     /mol_type="other DNA"
                     /isolation_source="personal collection, William Jacobs Jr.
                     and Sunhee Lee"
                     /db_xref="taxon:1552164"
     regulatory      1..363
                     /regulatory_class="promoter"
                     /gene="HSP60"
     gene            1..363
                     /gene="HSP60"
                     /label=HSP60
     CDS             517..2808
                     /codon_start=1
                     /product="Ag85ss-gp120ss-HIV 1086c gp120-siinfekl"
                     /label=Ag85ss-gp120ss-HIV 1086c gp120-siinfekl
                     /protein_id="AIS72850.1"
                     /translation="MRVRGIWKNWPQWLIWSILGFWIGNMEGSWVTVYYGVPVWKEAKT
                     TLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMVLANVTENFNMWKNDMVEQMHEDII
                     SLWDESLKPCVKLTPLCVTLNCTNVKGNESDTSEVMKNCSFKATTELKDKKHKVHALFY
                     KLDVVPLNGNSSSSGEYRLINCNTSAITQACPKVSFDPIPLHYCAPAGFAILKCNNKTF
                     NGTGPCRNVSTVQCAHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVHLNESV
                     NIVCTRPNNNTRKSIRIGPGQTFYATGDIIGNIRQAHCNINESKWNNTLQKVGEELAKH
                     FPSKTIKFEPSSGGDLEITTHSFNCRGEFFYCNTSDLFNGTYRNGTYNHTGRSSNGTIT
                     LQCKIKQIINMWQEVGRAIYAPPIEGEITCNSNITGLLLLRDGGQSNETNDTETFRPGG
                     GDMRDNWRSELYKYKVVEIKPLGVAPTEAKERVVEREKEAVGNVNIAIGGAATGIAAVL
                     TDGNPPEVKSVGLGNVNGVTLGYTSGTGQGNASATKDGSHYKITGTATGVDMANPMSPV
                     NKSFGIEVTCLEQLESIINFEKLTEWTSFEFAKFASFEAQGALKGSYGSTDADIRETAH
                     PGRKGLGRPRRGAWRGRGHRQRARSDLHRPPSRPRGHQPAGLRRAAAGRTSRAAARSDD
                     RHRGPQRADHRHRQADRRPGFAHPGRDIAAQLRGIRHPPASHGRRRTGHRSHHRPAAGP
                     HPARDDRGVR"
     CDS             2384..3838
                     /codon_start=1
                     /gene="leuCD"
                     /product="LeuCD"
                     /label=leuCD
                     /note="derived from Mycobacterium smegmatis"
                     /protein_id="AIS72851.1"
                     /translation="MDQPTQTSVKPRTLAEKVWDDHVVARGEGEGTAKEPDLIYIDLHL
                     VHEVTSPQAFDGLRLAGRPVRRPDLTIATEDHNVPTIDIDKPIADPVSRTQVETLRRNC
                     EEFGIRLHPMGDAEQGIVHIIGPQLGLTQPGMTVVCGDSHTSTHGAFGAIAMGIGTSEV
                     EHVMATQTLSLKPFKTMAVNVDGELPPGVSAKDIILAVIAKIGTGGGQGHVIEYRGSAI
                     RSLSMEGRMTICNMSIEAGARAGMVAPDETTFEFLKGRPHAPKGADWDAAVAAWSRLRT
                     DEGAEFDTEVYIDASTLSPFVTWGTNPGQGVPLSEAVPDPELIVDEGARQAAEKALAYM
                     DLRPGTPMRDIAVDTVFVGSCTNGRIEDLRVVADVLRGRKVADGVRMLIVPGSMRVRAQ
                     AESEGLDQIFTAAGAQWRQAGCSMCLGMNPDQLSPGERCASTSNRNFEGRQGKGGRTHL
                     VSPAVAAATAVRGKLASPADLPAAAR"
     gene            2384..3838
                     /gene="leuCD"
                     /label=leuCD
     rep_origin      4757..5156
                     /label=oriE
                     /note="oriE"
     rep_origin      4824..5412
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5812..7166
                     /label=oriM
                     /note="oriM"