LOCUS       Exported               10450 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Activation tagging vector pSKI074, complete sequence.
ACCESSION   AF218466
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Weigel D, Ahn JH, Blazquez MA, Borevitz JO, Christensen SK, 
            Fankhauser C, Ferrandiz C, Kardailsky I, Malancharuvil EJ, Neff MM, 
            Nguyen JT, Sato S, Wang Z, Xia Y, Dixon RA, Harrison MJ, Lamb CJ, 
            Yanofsky MF, Chory J.
  TITLE     Activation tagging in Arabidopsis
  JOURNAL   Plant Physiol. 122 (4), 1003-1013 (2000)
  PUBMED    10759496
REFERENCE   2  (bases 1 to 10450)
  AUTHORS   Weigel D, Blazquez MA, Borevitz J, Christensen SK, Fankhauser C, 
            Ferrandiz C, Malancharuvil EJ, Neff MM, Nguyen JT, Sato S, Xia Y, 
            Wang Z, Dixon RA, Harrison MJ, Lamb CJ, Yanofsky MF, Chory J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-1999) Plant Biology Laboratory, The Salk Institute
            for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 
            92037, USA
REFERENCE   3  (bases 1 to 10450)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10450
                     /organism="Activation tagging vector pSKI074"
                     /mol_type="other DNA"
                     /db_xref="taxon:112458"
     regulatory      12..31
                     /regulatory_class="promoter"
                     /note="from T7 RNA polymerase"
     promoter        complement(13..31)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    36..2204
                     /label=derived from pUC19
                     /note="derived from pUC19"
     primer_bind     complement(52..68)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    76..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(100..130)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    145..166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(454..1042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1213..2073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1662..2205
                     /label=derived from phage f1
                     /note="derived from phage f1"
     promoter        complement(2074..2178)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2207..2662)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    2663..2829
                     /label=derived from pUC19
                     /note="derived from pUC19"
     primer_bind     2807..2823
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2830..2849
                     /regulatory_class="promoter"
                     /note="from T7 RNA polymerase"
     promoter        2830..2848
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2881..2897
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2894..4178
                     /regulatory_class="enhancer"
                     /note="from cauliflower mosaic virus 35S gene promoter"
     misc_feature    4270..4294
                     /label=T-DNA right border
                     /note="T-DNA right border"
     oriT            5497..5606
                     /note="incP origin of transfer"
     rep_origin      6401..7113
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    7700..7724
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     primer_bind     complement(8045..8061)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8054..8784
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    8064..8749
                     /note="includes mannopine synthase promoter and 5 UTR 
                     sequence"
     promoter        8362..8741
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     mRNA            8756..9739
                     /product="neomycin phosphotransferase"
                     /label=neomycin phosphotransferase mRNA
     CDS             8771..9565
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /function="confers kanamycin resistance"
                     /label=neomycin phosphotransferase
                     /protein_id="AAF26422.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      9746..10430
                     /regulatory_class="terminator"
                     /note="from mannopine synthase"