LOCUS       Exported                5607 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pSK(+)PICT1, complete sequence.
ACCESSION   EU196039
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 5607)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Phagemid vector pSK(+)PICT1"
                     /mol_type="other DNA"
                     /note="used for the expression of cDNA as double-stranded 
                     hairpin RNA for post transcriptional gene silencing in Ulva
                     mutabilis"
                     /db_xref="taxon:492499"
     regulatory      1..546
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
     repeat_region   558..668
                     /label=derived from Escherichia coli transposon Tn5
                     /note="derived from Escherichia coli transposon Tn5"
     intron          680..842
                     /number=1
                     /note="derived from Ulva RbcS"
     CDS             complement(987..1646)
                     /codon_start=1
                     /gene="Cm"
                     /gene_synonym="CAT"
                     /product="chloramphenicol acetyl transferase"
                     /label=Cm
                     /note="Cm resistance gene from pBR328"
                     /protein_id="ABY60797.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(987..1646)
                     /gene="Cm"
                     /gene_synonym="CAT"
                     /label=Cm
     promoter        complement(1647..1749)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     intron          1843..2175
                     /number=1
                     /note="derived from Ulva RbcS"
     repeat_region   2182..2293
                     /label=derived from Escherichia coli transposon Tn5
                     /note="derived from Escherichia coli transposon Tn5"
     regulatory      2301..2689
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
     misc_feature    2689..5607
                     /note="derived from cloning vector pBlueScribe SK(+) with 
                     minor modifications"
     primer_bind     complement(2705..2721)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2747..2765)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2772..2788)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2933..3388
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3417..3521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3522..4382
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4553..5141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5429..5450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5465..5495
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5503..5519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5527..5543
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5564..5582
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"