LOCUS       Exported                6700 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pSix1 plasmid pTT8 DNA, complete sequence.
ACCESSION   LC428096
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Fujino Y, Doi K.
  TITLE     Development of a new gene expression vector for Thermus thermophilus
            using a silica-inducible promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Fujino Y, Doi K.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2018) Contact:Yasuhiro Fujino Kyushu University, 
            Institute of Genetic Resources; 744 Motooka Nishi-ku, Fukuoka, 
            Fukuoka 819-0395, Japan
REFERENCE   3  (bases 1 to 6700)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Expression vector pSix1"
                     /mol_type="other DNA"
                     /db_xref="taxon:2485480"
     CDS             complement(103..852)
                     /codon_start=1
                     /gene="TTHC004"
                     /product="hypothetical protein"
                     /label=TTHC004
                     /note="similar to DBJ:BAD12125.1 percent identity 100 in 
                     249 aa"
                     /protein_id="BBG91894.1"
                     /translation="MGQNHAIGCGPVSVLRILEVYRQRGLEGIPWDITLRLPKDDLSFS
                     GYSVTVPGSARIPSGLPQWAQLALWPVKLTTQNGKNIYNAWISQALNGKQAGLDQAVLP
                     KDYAPGANRWLADNGLPVRVTGAYLRDFDASFLLNPYIPITGPIAWVSYTQYTWTTNGL
                     LKDTIGNRDLPAVVLYDSAQIRSGSSYYLHYAPAYRYRVVEYWDWSANFATVDNGKEGL
                     SPRPFGPIEIYLSDYYDLSNGVWRISR"
     gene            complement(103..852)
                     /gene="TTHC004"
                     /label=TTHC004
     CDS             complement(873..1379)
                     /codon_start=1
                     /gene="TTHC003"
                     /product="hypothetical protein"
                     /label=TTHC003
                     /note="similar to GB:EAK83744.1 percent identity 24 in 162 
                     aa"
                     /protein_id="BBG91895.1"
                     /translation="MYSSAFKESSLSATLAASPGSSGRSTGSWEAMREAHRKNGFLDEL
                     AKRWDRLIEKSPPVPSPQGLSPQSLGGDPKPLDLATEFFRDQVSPQATLPGQCLSWFLW
                     ACTAYRNAGYVSFTASTNTLPGDFGNQQNISYPWYWRGTPKQVYTNPLNAPSRATPTTP
                     GEPGT"
     gene            complement(873..1379)
                     /gene="TTHC003"
                     /label=TTHC003
     rep_origin      2281..3444
                     /note="ori; derived from Thermus thermophilus HB8 plasmid 
                     pTT8 (INSD:AP008228.1)"
     CDS             complement(2281..3444)
                     /codon_start=1
                     /gene="TTHC001"
                     /product="putative RepA protein"
                     /label=TTHC001
                     /note="similar to DBJ:BAD12124.1 percent identity 100 in 
                     387 aa"
                     /protein_id="BBG91896.1"
                     /translation="MVLRAYAALRGLSPEALRAHLLAPPLRPERAREAFQRPYLAHFAQ
                     TLPRYPYATDDPKEGVRIYKRENALKRVHVQVGHYPHAVLRLVVDVDLPWPQVEERIHA
                     LPPSLVLVNPRSGHFHAWYELDPIPLTPPPGREGSLKGALALLAEVEALLEAYYGADPG
                     YNGLLSRNPFLHPPEWTWGGGKRWSLRDLHRELRGLLPSGTRRRVDPGLASYGRNNALF
                     DRLRAEAYAHVALFRGVPGGEEAFRAWVEQRAHALNQSLFRDHPKGPLDPREVHHTAKS
                     VAKWTYRNYRGARVYPVSSTGRPDRSRLSPQARALIPPLQGQELQEAVREGGRRRGSRR
                     RQEAEEKLTEALKRLQARGERVTARALAREAGVKPHTASKWLKRMRE"
     gene            complement(2281..3444)
                     /gene="TTHC001"
                     /label=TTHC001
     CDS             complement(3677..4114)
                     /codon_start=1
                     /gene="TTHC014"
                     /product="hypothetical protein"
                     /label=TTHC014
                     /note="similar to GB:AAS81137.1 percent identity 38 in 118 
                     aa"
                     /protein_id="BBG91897.1"
                     /translation="MEHPEGEDPTLLPPALAARALGVSPATLRRYAALWERLVGPLPRD
                     PRGGRLWPKEALARLRAAREAHLREGLPLEEALARVQGDFPALALPSEGEALALLRALA
                     ERLERVEGELRALREENARLREALKALEPPRRRPWWRFWGH"
     gene            complement(3677..4114)
                     /gene="TTHC014"
                     /label=TTHC014
     CDS             complement(4197..4466)
                     /codon_start=1
                     /gene="TTHC013"
                     /label=TTHC013
                     /note="similar to DBJ:BAD12123.1 percent identity 98 in 89 
                     aa"
                     /protein_id="BBG91898.1"
                     /translation="MGYRIEFDPRAEKELEKLDREVARRILRFLRERVATLEDPRSLGE
                     PLRGPELGRFWKYRVGDYRLICHIQDREATVLVLRVGHRRDVYR"
     gene            complement(4197..4466)
                     /gene="TTHC013"
                     /label=TTHC013
     CDS             complement(4450..4662)
                     /codon_start=1
                     /gene="TTHC012"
                     /product="anti-toxin-like protein"
                     /label=TTHC012
                     /note="similar to DBJ:BAD12122.1 percent identity 100 in 70
                     aa"
                     /protein_id="BBG91899.1"
                     /translation="MVVVRLPKEIEERLTALARKTGRSKSYYVRQALLEYLDDLEDYYL
                     AVERLEERLPGIPLDEVERRLGLSD"
     gene            complement(4450..4662)
                     /gene="TTHC012"
                     /label=TTHC012
     regulatory      4882..5478
                     /regulatory_class="promoter"
                     /note="derived from Thermus thermophilus HB8"
     CDS             5482..5514
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             5564..5581
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5648..5695
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5713..6621)
                     /codon_start=1
                     /product="hygromycin phosphotransferase"
                     /label=hygromycin phosphotransferase
                     /note="thermostable mutant of the E. coli hygromycin 
                     phosphotransferase which has 20 amino-acid substitutions 
                     and confers hygromycin resistance to Thermus thermophilus 
                     at 81C"
                     /protein_id="BBG91900.1"
                     /translation="MKKPELTVTSVEKFLIEKFGSVSHLMQLSEGEESRAFSFDVGGCG
                     YVLRVSSRADGFYKDRYVYQHFASAALPIPEVLDIGEFSEGLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEVMDAIAAADLSQTSGFGPFGPQGIGQYATWRDFICAIADPHVYDWQ
                     TVMDDTVSASVARALDELMLWAEDCPEVRHLVHGDFGSNNVLTDNGRITAVIDWSEAMF
                     GDPLYEVANIFFWRPWLACMEQQARYFERRHPELARSPRLRAYMLRIGLDQLYQSLVNG
                     NFEDAAWAQSRCDAIVRSGAG"
     regulatory      6621..6695
                     /regulatory_class="promoter"
                     /note="promoter region of Thermus aquaticus YT1 isocitrate 
                     dehydrogenase gene"