LOCUS       Exported                4364 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector psiSTRIKE Puromycin, complete sequence.
ACCESSION   AY497507
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4364)
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Cloning vector psiSTRIKE Puromycin"
                     /mol_type="other DNA"
                     /db_xref="taxon:258975"
     regulatory      16..275
                     /regulatory_class="promoter"
                     /note="U6"
     promoter        27..267
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     regulatory      330..349
                     /regulatory_class="promoter"
                     /note="SP6 RNA polymerase"
     promoter        complement(331..349)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(367..388)
                     /label=pUC/M13 reverse sequencing primer
                     /note="pUC/M13 reverse sequencing primer"
     primer_bind     complement(367..383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    391..407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(415..445)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    460..481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    601..1019
                     /label=SV40 enhancer and early promoter
                     /note="SV40 enhancer and early promoter"
     promoter        656..1013
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      864..999
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      917..982
                     /label=SV40 minimum origin of replication
                     /note="SV40 minimum origin of replication"
     CDS             1042..1641
                     /codon_start=1
                     /product="puromycin-N-acetyltransferase"
                     /label=puromycin-N-acetyltransferase
                     /protein_id="AAR91728.1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     regulatory      1686..1734
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic"
     polyA_signal    1686..1734
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(1927..2515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2686..3546)
                     /codon_start=1
                     /product="b-lactamase"
                     /label=b-lactamase
                     /note="AmpR"
                     /protein_id="AAR91727.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3547..3651)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3729..4184)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4298..4321
                     /label=pUC/M13 forward sequencing primer
                     /note="pUC/M13 forward sequencing primer"
     primer_bind     4325..4341
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4348..3
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase"
     promoter        4348..2
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"