LOCUS       Exported                3632 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSI, mammalian expression vector, complete sequence.
ACCESSION   U47121
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Brondyk WH.
  TITLE     pCI and pSI Mammalian Expression Vectors
  JOURNAL   Promega Notes 49, 7-11 (1994)
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Brondyk WH, Groskreutz DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-1996) Melinda Brady, R&D, Promega Corporation, 
            5445 East Cheryl Parkway, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 3632)
  AUTHORS   Brondyk WH, Groskreutz DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-1999) Melinda Brady, R&D, Promega Corporation, 
            5445 East Cheryl Parkway, Madison, WI 53711, USA
REFERENCE   4  (bases 1 to 3632)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Sep 13, 1999 this sequence version replaced U47121.1.
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Cloning vector pSI"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:45773"
     regulatory      1..419
                     /regulatory_class="promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        56..413
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      264..399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          483..615
                     /note="chimeric intron"
     regulatory      660..678
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        660..678
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    677
                     /label=T7 promoter transcription start site
                     /note="T7 promoter transcription start site"
     misc_feature    678..730
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     regulatory      737..958
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     polyA_signal    746..867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1048..1503
                     /label=phage f1 region
                     /note="phage f1 region"
     promoter        1835..1939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1940..2800
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /label=ampR
                     /protein_id="AAA88783.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            1940..2800
                     /gene="ampR"
                     /label=ampR
     rep_origin      2971..3559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"