LOCUS       Exported                6568 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  AdEasy vector pShuttle, complete sequence.
ACCESSION   AF334399
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Grafsky AJ III.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2001) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="AdEasy vector pShuttle"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="extrachromosomal used for the cloning of cDNA to be 
                     recombined with the pADEasy-1 vector; for infection of, and
                     expression in, various mammalian cell types"
                     /db_xref="taxon:148367"
     repeat_region   4..106
                     /label=left inverted terminal repeat (LITR)
                     /note="left inverted terminal repeat (LITR)"
     misc_signal     170..320
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     regulatory      197..345
                     /regulatory_class="other"
                     /note="encapsidation"
     primer_bind     313..337
                     /label=forward
                     /note="forward"
     misc_feature    359..418
                     /label=MCS
                     /note="MCS"
     misc_feature    416..2414
                     /label=right arm homology region
                     /note="right arm homology region"
     primer_bind     452..473
                     /label=reverse
                     /note="reverse"
     misc_feature    2682..3583
                     /label=left arm homology region
                     /note="left arm homology region"
     repeat_region   3584..3686
                     /label=right inverted terminal repeat (RITR)
                     /note="right inverted terminal repeat (RITR)"
     rep_origin      3764..4542
                     /label=pBR322 ori
                     /note="pBR322 ori"
     rep_origin      complement(3888..4476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5289..6083
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /label=neomycin phosphotransferase
                     /note="kanamycin resistance protein"
                     /protein_id="AAK01721.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(6131..6510)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     repeat_region   6548..82
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"