LOCUS       Exported                5607 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pShuttle-SN, complete sequence.
ACCESSION   AY862402
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Liu RY, Wu LZ, Huang BJ, Huang JL, Zhang YL, Ke ML, Wang JM, Tan WP,
            Zhang RH, Chen HK, Zeng YX, Huang W.
  TITLE     Adenoviral expression of a truncated S1 subunit of SARS-CoV spike 
            protein results in specific humoral immune responses against 
            SARS-CoV in rats
  JOURNAL   Virus Res. 112 (1-2), 24-31 (2005)
  PUBMED    16022898
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Liu R-Y., Huang B-J., Wu L-Z., Huang J-L., Zhang R-H., Zeng Y-X., 
            Huang W.
  TITLE     Constructing recombinant adenovirus carrying the spike gene 
            fragments as a vaccine against SARS-CoV by in vitro ligation
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5607)
  AUTHORS   Liu R-Y., Huang B-J., Wu L-Z., Huang J-L., Zhang R-H., Zeng Y-X., 
            Huang W.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2004) Cancer Center, Sun Yat-Sen University, 651 
            Dongfeng Road East, Guangzhou, Guangdong 510060, China
REFERENCE   4  (bases 1 to 5607)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Expression vector pShuttle-SN"
                     /mol_type="other DNA"
                     /country="China"
                     /db_xref="taxon:308969"
     enhancer        262..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        890..908
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             990..2507
                     /codon_start=1
                     /product="truncated SARS coronavirus spike glycoprotein S1 
                     subunit"
                     /label=truncated SARS coronavirus spike glycoprotein S
                     /protein_id="AAW56614.1"
                     /translation="MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDE
                     IFRSDTLYLTQDLFLPFYSNVTGFHTINHTFDNPVIPFKDGIYFAATEKSNVVRGWVFG
                     STMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEY
                     ISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKL
                     PLGINITNFRAILTAFSPAQDTWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQN
                     PLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAW
                     EGKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAP
                     GQTGVIADYNYKLPDDFMGCVLAWNTRNIDATPTGNYNYKYRYLRHGKLRPFERDISNV
                     PFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGTKLKFKPLISLDCAF"
     misc_feature    990..2459
                     /label=Region: SARS coronavirus spike glycoprotein
                     /note="Region: SARS coronavirus spike glycoprotein"
     misc_feature    2460..2507
                     /label=derived from pShuttle vector
                     /note="derived from pShuttle vector"
     polyA_signal    2495..2719
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3055..3643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4340..5155)
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"