LOCUS       Exported                4614 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSGHV0, complete sequence.
ACCESSION   AF285183
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Leahy DJ, Dann CE III, Longo P, Perman B, Ramyar KX.
  TITLE     A mammalian expression vector for expression and purification of 
            secreted proteins for structural studies
  JOURNAL   Protein Expr. Purif. 20 (3), 500-506 (2000)
  PUBMED    11087690
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Leahy DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2000) Biophysics, Johns Hopkins University, 725 N.
            Wolfe St., Baltimore, MD 21205, USA
REFERENCE   3  (bases 1 to 4614)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Cloning vector pSGHV0"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:148881"
     misc_feature    1..4614
                     /note="mammalian expression vector; directs expression of 
                     hGH fusion proteins"
     promoter        18..631
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             363..631
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             join(700..709,970..1130,1340..1459,1552..1716,1969..2280)
                     /codon_start=1
                     /product="human growth hormone/octahistidine/etch virus 
                     protease recognition site fusion protein precursor"
                     /label=human growth hormone/octahistidine/etch virus p
                     /protein_id="AAG59857.1"
                     /translation="MATGSRTSLLLAFGLLCLPWLQEGSAFPTIPLSRLFDNAMLRAHR
                     LHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRI
                     SLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQ
                     IFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGFSGHHH
                     HHHHHDYDIPSSENLYFQGSSRARTRAAARGHA"
     misc_feature    join(700..709,970..1130,1340..1459,1552..1716,1969..2139)
                     /label=Region: human growth hormone precursor
                     /note="Region: human growth hormone precursor"
     sig_peptide     join(700..709,970..1037)
     mat_peptide     join(1038..1130,1340..1459,1552..1716,1969..2277)
                     /product="human growth hormone/octahistidine/etch virus 
                     protease recognition site fusion protein"
     misc_feature    2140..2163
                     /label=Region: octahistidine tag
                     /note="Region: octahistidine tag"
     misc_feature    2164..2205
                     /note="Region: tobacco etch virus protease recognition 
                     site"
     CDS             2170..2193
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             2215..2235
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_feature    2233..2284
                     /label=multiple cloning site
                     /note="multiple cloning site"
     polyA_signal    2286..2420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2747..3335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3506..4366)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AAG59856.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4367..4471)
                     /gene="bla"
                     /label=AmpR promoter