LOCUS       Exported                4773 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pSGFPS1, complete sequence.
ACCESSION   MF769790
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Rodriguez MD, Paul Z, Wood CE, Rice KC, Triplett EW.
  TITLE     Construction of Stable Fluorescent Reporter Plasmids for Use in 
            Staphylococcus aureus
  JOURNAL   Front Microbiol 8, 2491 (2017)
  PUBMED    29312199
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Rodriguez MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2017) Microbiology and Cell Science, University of
            Florida, 1355 Museum Rd., PO Box 110700, Gainesville, FL 32611-0700,
            USA
REFERENCE   3  (bases 1 to 4773)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Reporter vector pSGFPS1"
                     /mol_type="other DNA"
                     /db_xref="taxon:2073111"
     CDS             1..996
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /label=repF
                     /protein_id="AUS76872.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            1..996
                     /gene="repF"
                     /label=repF
     rep_origin      1147..1570
                     /label=R6K origin
                     /note="R6K origin"
     rep_origin      1179..1567
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /regulatory_class="promoter"
                     /note="sarA P1 promoter"
     CDS             1847..2332
                     /codon_start=1
                     /gene="dfrA"
                     /product="DfrA"
                     /label=dfrA
                     /protein_id="AUS76873.1"
                     /translation="MTLSIIVAHDKQRVIGYQNQLPWHLPNDLKHIKQLTTGNTLVMAR
                     KTFNSIGKPLPNRRNVVLTNQASFRHEGVDVINSLDEIKELSGHVFIFGGQTLYEAMID
                     QVDDMYITVIDGKFQGDTFFPPYTFENWEVESSVEGQLDEKNTIPHTFLHLVRRKGK"
     gene            1847..2332
                     /gene="dfrA"
                     /label=dfrA
     CDS             2340..3062
                     /codon_start=1
                     /gene="sfGFP"
                     /product="green fluorescent protein"
                     /label=sfGFP
                     /protein_id="AUS76874.1"
                     /translation="MPGSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKD
                     DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGI
                     KANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVL
                     LEFVTAAGITHGMDELYK"
     gene            2340..3062
                     /gene="sfGFP"
                     /label=sfGFP
     CDS             2349..3059
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     regulatory      3459..3516
                     /regulatory_class="terminator"
                     /note="atl transcription terminator"
     rep_origin      3913..4090
                     /label=sso
                     /note="sso"
     misc_feature    4559..4579
                     /label=dso
                     /note="dso"