LOCUS       Exported                6444 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integrating GFP expression vector pSG-CaGFP-1, complete sequence.
ACCESSION   FJ859897
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Galuska S, Donald RGK., Muise E, Wiersma HI, Diaz C, Kulkarni A, 
            Cavet G, Roemer T, Liberator P, Douglas C.
  TITLE     A Candida albicans GFP reporter assay for identifying inhibitors of 
            cell wall biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Galuska S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2009) Infectious Diseases, Merck & Co., 126 E. 
            Lincoln Ave, E. Lincoln Ave., NJ 07079, USA
REFERENCE   3  (bases 1 to 6444)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Integrating GFP expression vector pSG-CaGFP-1"
                     /lab_host="Candida albicans"
                     /mol_type="other DNA"
                     /db_xref="taxon:741972"
     CDS             241..1653
                     /codon_start=1
                     /product="ARG4"
                     /label=ARG4
                     /note="contains unique NotI site"
                     /protein_id="ADD22644.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     AADAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEP
                     LFDALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHI
                     SGECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAV
                     LKQLENLKSILS"
     misc_feature    1081..1088
                     /note="NotI site; inserted in ARG4 to facilitate targeted 
                     integration at the ARG4 locus of Candida albicans 
                     transformants"
     primer_bind     1910..1926
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1963..2679
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=green fluorescent protein
                     /note="codon-optimized; same codon-optimized GFP as in 
                     Cormack et al (1997) Microbiology 143: 303-311"
                     /protein_id="ADD21571.1"
                     /translation="LINGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2864..2880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2888..2904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2912..2942)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2957..2978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3266..3854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4025..4885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4886..4990)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5435..6247
                     /codon_start=1
                     /product="URA3"
                     /label=URA3
                     /note="selectable marker"
                     /protein_id="ADD21572.1"
                     /translation="MTVNTKTYSERAETHASPVAQRLFRLMELKKTNLCASIDVDTTKE
                     FLELIDKLGPYVCLIKTHIDIINDFSYESTIEPLLELSRKHQFMIFEDRKFADIGNTVK
                     KQYIGGVYKISSWADITNAHGVTGNGVVEGLKQGAKETTTNQEPRGLLMLAELSSVGSL
                     AYGEYSQKTVEIAKSDKEFVIGFIAQRDMGGQEEGFDWLIMTPGVGLDDKGDGLGQQYR
                     TVDEVVSTGTDIIIVGRGLFGKGRDPDIEGKRYRNAGWNAYLKKTGQL"