LOCUS       Exported               11028 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSF_lenti_ZEBOV14_NP-VP35-GP, complete sequence.
ACCESSION   KT186367
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Mattiuzzo G, Ashall J, Doris KS, MacLellan-Gibson K, Nicolson C, 
            Wilkinson DE, Harvey R, Almond N, Anderson R, Efstathiou S, Minor 
            PD, Page M.
  TITLE     Development of Lentivirus-Based Reference Materials for Ebola Virus 
            Nucleic Acid Amplification Technology-Based Assays
  JOURNAL   PLoS ONE 10 (11), E0142751 (2015)
  PUBMED    26562415
REFERENCE   2  (bases 1 to 11028)
  AUTHORS   Mattiuzzo G, Mitchell J, Mee ET.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2015) Virology, National Institute for Biological 
            Standards and Controls, Blanche Lane, South Mimms-Potters Bar EN6 
            3QG, United Kingdom
REFERENCE   3  (bases 1 to 11028)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Geneious v. 7.1.8
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Cloning vector pSF_lenti_ZEBOV14_NP-VP35-GP"
                     /mol_type="other DNA"
                     /note="modified Zaire Ebolavirus 
                     H.sapiens-wt/GIN/2014/Makona-Kissidougou-C15 sequence in 
                     pSF_lenti backbone"
                     /db_xref="taxon:1675614"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     601..617
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        713..1016
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1018..1220
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1235..1415
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     misc_feature    1462..1587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2080..2313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2809..2925
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3001..3092
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     gene            complement(3097..5280)
                     /gene="NP"
                     /label=NP
     gene            complement(5284..6153)
                     /gene="VP35"
                     /label=VP35
     gene            complement(6285..8291)
                     /gene="GP"
                     /label=GP
     LTR             8386..8619
                     /label=3 LTR (Delta-U3)
                     /note="self-inactivating 3 long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8636..8757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(8840..8858)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8879..8895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8903..8919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8927..8957)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8972..8993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9281..9869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10040..10900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10901..11005)
                     /gene="bla"
                     /label=AmpR promoter