LOCUS       Exported                4543 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pSFMI-lib, complete sequence.
ACCESSION   HM356026
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Kocsis A, Kekesi KA, Szasz R, Vegh BM, Balczer J, Dobo J, Zavodszky 
            P, Gal P, Pal G.
  TITLE     Selective Inhibition of the Lectin Pathway of Complement with Phage 
            Display Selected Peptides against Mannose-Binding Lectin-Associated 
            Serine Protease (MASP)-1 and -2: Significant Contribution of MASP-1 
            to Lectin Pathway Activation
  JOURNAL   J. Immunol. 185 (7), 4169-4178 (2010)
  PUBMED    20817870
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Kocsis A, Kekesi KA, Szasz R, Vegh BM, Balczer J, Dobo J, Zavodszky 
            P, Gal P, Pal G.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2010) Institute of Enzymology, BRC Hungarian 
            Academy of Sciences, Karolina ut 29., Budapest H-1113, Hungary
REFERENCE   3  (bases 1 to 4543)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Phagemid vector pSFMI-lib"
                     /mol_type="other DNA"
                     /db_xref="taxon:888514"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     gene            128..989
                     /gene="bla"
                     /label=bla
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ADN85857.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1875..1952
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             1953..3035
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             2076..3035
                     /codon_start=1
                     /gene="lacIq"
                     /product="lac repressor"
                     /label=lacIq
                     /protein_id="ADN85858.1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA
                     LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR
                     VGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVS
                     LVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"
     gene            2076..3035
                     /gene="lacIq"
                     /label=lacIq
     protein_bind    3048..3069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3278..3306
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    3314..3330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3400..3858
                     /codon_start=1
                     /product="malE-signal/SFMI/FLAG/SGCI/p8 casette ORF"
                     /label=malE-signal/SFMI/FLAG/SGCI/p8 casette ORF
                     /protein_id="ADN85859.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAG?C????PP?C?PDGGSGG
                     SGGDYKDDDDKLEVTCEPGTTFKDKCNTCRCGSDGKSAACTLKACPQGTGGGSGSSSAE
                     GDDPAKAAFNSLQASATEYIGYAWAMVVVIVGATIGIKLFKKFTSKAS"
     misc_feature    3400..3519
                     /label=malE-signal encoding region
                     /note="malE-signal encoding region"
     misc_feature    3520..3543
                     /label=linker
                     /note="linker"
     misc_feature    3544..3567
                     /label=FLAG epitope encoding region
                     /note="FLAG epitope encoding region"
     CDS             3544..3567
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3571..3675
                     /label=SGCI encoding region
                     /note="SGCI encoding region"
     misc_feature    3676..3705
                     /label=linker
                     /note="linker"
     misc_feature    3706..3855
                     /label=p8 protein encoding region
                     /note="p8 protein encoding region"
     promoter        complement(3900..3918)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3928..3944)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(4085..4540)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"