LOCUS       Exported                4506 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Thermosensitive suicide vector pSET4s DNA, complete sequence.
ACCESSION   AB055650
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Thermosensitive suicide vectors for gene replacement in 
            Streptococcus suis
  JOURNAL   Plasmid 46 (2), 140-148 (2001)
  PUBMED    11591139
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2001) Daisuke Takamatsu, National Institute of 
            Animal Health, Laboratory of Molecular Bacteriology; Kannondai 
            3-1-1, Tsukuba, Ibaraki 305-0856, Japan 
            (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, 
            Fax:81-298-38-7743)
REFERENCE   3  (bases 1 to 4506)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Thermosensitive suicide vector pSET4s"
                     /mol_type="other DNA"
                     /db_xref="taxon:152114"
     CDS             complement(133..144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             528..689
                     /codon_start=1
                     /gene="orfC"
                     /product="ORFC"
                     /label=orfC
                     /protein_id="BAB64880.1"
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
     gene            528..689
                     /gene="orfC"
                     /label=orfC
     CDS             756..1454
                     /codon_start=1
                     /gene="repAts"
                     /product="RepAts"
                     /label=repAts
                     /protein_id="BAB64881.1"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     gene            756..1454
                     /gene="repAts"
                     /label=repAts
     CDS             complement(1803..2126)
                     /codon_start=1
                     /gene="lacZ"
                     /product="LacZ"
                     /label=lacZ
                     /protein_id="BAB64882.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     gene            complement(1803..2126)
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     2036..2052
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2053..2109
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2122..2138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2146..2162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2170..2200)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2215..2236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2524..3112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3637..4404
                     /codon_start=1
                     /gene="spc"
                     /product="spectinomycin adenyltransferase"
                     /label=spc
                     /protein_id="BAB64883.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            3637..4404
                     /gene="spc"
                     /label=spc