LOCUS       Exported                5016 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pSET2 DNA, complete sequence.
ACCESSION   AB042430
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Construction and characterization of Streptococcus suis-Escherichia 
            coli shuttle cloning vectors
  JOURNAL   Plasmid 45 (2), 101-113 (2001)
  PUBMED    11322824
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2000) Daisuke Takamatsu, National Institute of 
            Animal Health, Laboratory of Molecular Bacteriology; Kannondai 
            3-1-1, Tukuba, Ibaraki 305-0856, Japan 
            (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, 
            Fax:81-298-38-7743)
REFERENCE   3  (bases 1 to 5016)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Shuttle vector pSET2"
                     /lab_host="Streptococcus suis, Escherichia coli"
                     /mol_type="other DNA"
                     /label=synonym:Shuttle Cloning Vector pSET2
                     /note="synonym:Shuttle Cloning Vector pSET2"
                     /db_xref="taxon:125568"
     misc_feature    156..176
                     /gene="dso"
                     /label=double-stranded replication origin
                     /note="double-stranded replication origin"
     gene            156..176
                     /gene="dso"
                     /label=dso
     CDS             367..504
                     /codon_start=1
                     /gene="copG"
                     /product="CopG"
                     /function="transcriptional repressor protein"
                     /label=copG
                     /protein_id="BAB03239.1"
                     /translation="MKKRLTITLSESVLENLEKMAKEMGLSKSALISVALENYKKGQVK
                     "
     gene            367..504
                     /gene="copG"
                     /label=copG
     CDS             566..1246
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /function="The plasmid-encoded initiator of replication. 
                     The protein introduces a strand- and site-specific nick in 
                     the double-stranded replication origin, on supercoiled 
                     DNA."
                     /label=repB
                     /protein_id="BAB03240.1"
                     /translation="MAKEKARYFTFLLYPESIPSDWELKLELLGVPIAVSPLHDRDKSD
                     VEGQQYKKPHYHVIYVSKNPVTADSVRMKIKRSLGDNSVALVQIIRTSIENTYLYLTHE
                     SKDAIEKKKHVYDKADITLLSNFDIDRYITLDVEEKDDMLNEVCDLIDEYDIANMRELR
                     RFIKLHGAEHGLPSIKIINSVLRSHTGLIRLYFDAVYQERKYGSNIPNVNQETGEILSE
                     DSDD"
     gene            566..1246
                     /gene="repB"
                     /label=repB
     CDS             complement(1703..2470)
                     /codon_start=1
                     /gene="add9"
                     /product="spectinomycin adenyltransferase"
                     /function="spectinomycin resistance"
                     /label=add9
                     /protein_id="BAB03241.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            complement(1703..2470)
                     /gene="add9"
                     /label=add9
     CDS             complement(2872..3195)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase alpha fragment"
                     /label=lacZ
                     /protein_id="BAB03242.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     gene            complement(2872..3195)
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     3105..3121
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3122..3178
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3191..3207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3215..3231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3239..3269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3284..3305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4111..4252
                     /gene="ssoA"
                     /label=single-stranded replication origin
                     /note="single-stranded replication origin"
     gene            4111..4252
                     /gene="ssoA"
                     /label=ssoA