LOCUS       Exported                7382 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pSEmp18BBXM, complete sequence.
ACCESSION   U38693
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Nguyen TN, Gourdon M-H., Hansson M, Robert A, Samuelson P, Libon C, 
            Andreoni C, Nygren P-A., Stahl S.
  TITLE     Hydrophobicity engineering to facilitate surface display of 
            heterologous gene products on Staphylococcus xylosus
  JOURNAL   J. Biotechnol. (1997) In press
REFERENCE   2  (bases 1 to 7382)
  AUTHORS   Liljeqvist S, Samuelson P, Hansson M, Nguyen TN, Binz H, Stahl S.
  TITLE     Surface display of the cholera toxin B subunit on Staphylococcus 
            xylosus and Staphylococcus carnosus
  JOURNAL   Appl. Environ. Microbiol. 63 (7), 2481-2488 (1997)
  PUBMED    9212399
REFERENCE   3  (bases 1 to 7382)
  AUTHORS   Samuelson PN.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-1995) Biochemistry and Biotechnology, KTH, Royal 
            Institute of Technology, Teknikringen 34, Stockholm, 10044, Sweden
REFERENCE   4  (bases 1 to 7382)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Shuttle vector pSEmp18BBXM"
                     /mol_type="other DNA"
                     /label=functional in both E. coli and staphylococci
                     /note="functional in both E. coli and staphylococci"
                     /db_xref="taxon:64140"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..1061
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=bla
                     /protein_id="AAB67757.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            201..1061
                     /gene="bla"
                     /label=bla
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2284..2298)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             3787..4437
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /function="chloramphenicol resistance"
                     /label=cat
                     /protein_id="AAB67758.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            3787..4437
                     /gene="cat"
                     /label=cat
     promoter        5054..5084
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5092..5108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5116..5132
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5339..6745
                     /codon_start=1
                     /product="recombinant heterologous surface receptor"
                     /label=recombinant heterologous surface receptor
                     /note="contains the human serum albumin binding domain of; 
                     streptococcal protein G and the C-terminal cell wall 
                     anchoring region of staphylococcal protein A"
                     /protein_id="AAB67756.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAVDA
                     NSSSVPGDPLESTCRHASLDALAKAKADALKEFNKYGVSDYYKNLINNAKTVEGVKDLQ
                     AQVVESAKKARISEATDGLSDFLKSQTPAEDTVKSIELAEAKVLANRELDKYGVSDYHK
                     NLINNAKTVEGVKDLQAQVVESAKKARISEATDGLSDFLKSQTPAEDTVKSIELAEAKV
                     LANRELDKYGVSDYYKNLINNAKTVEGVKALIDEILAALPLESFAQAPKEEDNNKPGKE
                     DNNKPGKEDNNKPGKEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGN
                     KPGKEDNKKPGKEDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAA
                     DNKLADKNMIKPGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGA
                     ALLAGRRREL"
     sig_peptide     5339..5446
     misc_feature    5473..5539
                     /label=cloning region
                     /note="cloning region"
     misc_feature    5473..5529
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"