LOCUS       Exported                5595 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pSE1, complete sequence.
ACCESSION   KY615710
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Wu Z.
  TITLE     Single large serine recombinase mediates the horizontal transfer of 
            SCCmec in staphylococci
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2017) College of Animal Science & Technology, 
            Northwest A&F University, 22 St. Xinong, Yangling, Shaanxi 712100, 
            China
REFERENCE   3  (bases 1 to 5595)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Shuttle vector pSE1"
                     /mol_type="other DNA"
                     /db_xref="taxon:2022394"
     promoter        255..359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             360..1220
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ASN64765.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            360..1220
                     /gene="bla"
                     /label=bla
     misc_feature    1507..1647
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2437..3087)
                     /codon_start=1
                     /gene="cat(+)"
                     /product="chloramphenicol resistance protein"
                     /label=cat(+)
                     /protein_id="ASN64766.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(2437..3087)
                     /gene="cat(+)"
                     /label=cat(+)
     CDS             complement(3751..4755)
                     /codon_start=1
                     /gene="repB"
                     /product="RepB replication protein"
                     /label=repB
                     /protein_id="ASN64767.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     gene            complement(3751..4755)
                     /gene="repB"
                     /label=repB
     regulatory      5341..5439
                     /regulatory_class="promoter"
                     /note="PmecA"
     promoter        complement(5547..5565)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5572..5588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"