LOCUS       Exported                4439 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSDU1 deltamobdeltaorfEF, complete sequence.
ACCESSION   MF163399
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Wang R, Lin C, Lin J, Pang X, Liu X, Zhang C, Lin J, Chen L.
  TITLE     Construction of novel pJRD215-derived plasmids using chloramphenicol
            acetyltransferase (cat) gene as a selection marker for 
            Acidithiobacillus caldus
  JOURNAL   PLoS ONE 12 (8), e0183307 (2017)
  PUBMED    28813510
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Wang R, Chen L, Lin J, Lin J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2017) State Key Laboratory of Microbial 
            Technology, School of Life Sciences, Shandong University, 27 Shanda 
            South Road, Jinan, Shandong 250100, China
REFERENCE   3  (bases 1 to 4439)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit v. 7.1.3.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Cloning vector pSDU1 deltamobdeltaorfEF"
                     /mol_type="other DNA"
                     /db_xref="taxon:2171590"
     rep_origin      131..525
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             658..1629
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /label=repB
                     /protein_id="AWD72713.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     gene            658..1629
                     /gene="repB"
                     /label=repB
     CDS             1690..2529
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /protein_id="AWD72714.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     gene            1690..2529
                     /gene="repA"
                     /label=repA
     CDS             2516..3367
                     /codon_start=1
                     /gene="repC"
                     /product="RepC"
                     /label=repC
                     /protein_id="AWD72715.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     gene            2516..3367
                     /gene="repC"
                     /label=repC
     misc_feature    3561..3617
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             3706..4365
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="AWD72716.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            3706..4365
                     /gene="cat"
                     /label=cat