LOCUS       Exported                8786 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning shuttle vector pSCR119, complete sequence.
ACCESSION   AY622810
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Summers ML, Wallis JG, Campbell EL, Meeks JC.
  TITLE     Genetic evidence of a major role for glucose-6-phosphate 
            dehydrogenase in nitrogen fixation and dark growth of the 
            cyanobacterium Nostoc sp. strain ATCC 29133
  JOURNAL   J. Bacteriol. 177 (21), 6184-6194 (1995)
  PUBMED    7592384
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   Argueta C, Yuksek K, Summers M.
  TITLE     Construction and use of GFP reporter vectors for analysis of 
            cell-type-specific gene expression in Nostoc punctiforme
  JOURNAL   J. Microbiol. Methods 59 (2), 181-188 (2004)
  PUBMED    15369854
REFERENCE   3  (bases 1 to 8786)
  AUTHORS   Summers ML, Wallis JG, Campbell EL, Meeks JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St.,
            Northridge, CA 91330-8303, USA
REFERENCE   4  (bases 1 to 8786)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8786
                     /organism="Cloning shuttle vector pSCR119"
                     /mol_type="other DNA"
                     /db_xref="taxon:282192"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    212..268
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    218..261
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(281..297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    305..321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(329..359)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    374..395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      501..1381
                     /label=ColE1 origin of replication for use in E. coli
                     /note="ColE1 origin of replication for use in E. coli"
     rep_origin      complement(683..1271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1770..2564)
                     /codon_start=1
                     /gene="npt"
                     /product="neomycin phosphotransferase"
                     /function="neomycin and kanamycin resistance"
                     /label=npt
                     /protein_id="AAX31729.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(1770..2564)
                     /gene="npt"
                     /label=npt
     rep_origin      2926..8786
                     /label=pDC1 cyanobacterial origin of replication
                     /note="pDC1 cyanobacterial origin of replication"
     CDS             complement(8676..285)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase alpha subunit"
                     /label=lacZ
                     /protein_id="AAX31728.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRINSFVISAES
                     FERKPGRCIQVYQTYDESLKEESSESE"
     gene            complement(8676..285)
                     /gene="lacZ"
                     /label=lacZ