LOCUS       Exported                8596 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pSCPT DNA, complete sequence.
ACCESSION   AB871645
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Kanda K, Ishida T, Hirota R, Ono S, Motomura K, Ikeda T, Kitamura K,
            Kuroda A.
  TITLE     Application of a phosphite dehydrogenase gene as a novel dominant 
            selection marker for yeasts
  JOURNAL   J. Biotechnol. 182-183, 68-73 (2014)
  PUBMED    24786825
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Hirota R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2013) Contact:Ryuichi Hirota Hiroshima University,
            Department of Biotechnology; 1-3-1, Higashi-Hiroshima, Hiroshima 
            739-8530, Japan
REFERENCE   3  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Yeast expression vector pSCPT"
                     /focus
                     /strain="contains synthetic RsptxD DNA for improved 
                     expression in S. cerevisiae"
                     /mol_type="other DNA"
                     /db_xref="taxon:1430311"
     source          3695..3764
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /db_xref="taxon:4932"
     source          4776..5513
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /db_xref="taxon:4932"
     misc_feature    1..3480
                     /note="derived from YEp24 yeast extrachromosomal 
                     plasmid(L09156.1)"
     misc_feature    1..6
                     /label=EcoRI recognition site
                     /note="EcoRI recognition site"
     rep_origin      344..1686
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    676..723
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    2241..2246
                     /label=EcoRI recognition site
                     /note="EcoRI recognition site"
     promoter        2283..2498
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2499..3302
                     /codon_start=1
                     /gene="ura3"
                     /product="orotidine 5-phosphate decarboxylase"
                     /label=ura3
                     /protein_id="BAP74304.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            2499..3302
                     /gene="ura3"
                     /label=ura3
     misc_feature    2685..2690
                     /gene="ura3"
                     /label=EcoRV recognition site
                     /note="EcoRV recognition site"
     misc_feature    2873..2878
                     /gene="ura3"
                     /label=ApaI recognition site
                     /note="ApaI recognition site"
     misc_feature    3378..3383
                     /label=SmaI recognition site
                     /note="SmaI recognition site"
     regulatory      3695..3764
                     /regulatory_class="promoter"
                     /note="IPCS promoter"
     CDS             3765..4775
                     /codon_start=1
                     /gene="OPTptxD"
                     /product="phosphite dehydrogenase"
                     /label=OPTptxD
                     /protein_id="BAP74305.1"
                     /translation="MKPKVVLTHWVHPEIIELLSASADVIPNTTRETLPRSEVIARAKD
                     ADALMAFMPDSIDSAFLEECPKLRVIGAALKGYDNFDVNACTRHGVWLTIVPDLLTIPT
                     AELTIGLLLGLTRHMLEGDRQIRSGHFQGWRPTLYGSGLTGKTLGIIGMGAVGRAIAQR
                     LAGFEMNLLYCDPIPLNAEQEKAWHVQRVTLDELLEKCDYVVPMVPMAAETLHLIDATA
                     LAKMKTGSYLINACRGSVVDENAVIAALASGKLAGYAADVFEMEEWIRADRPQAIPKAL
                     LDNTAQTFFTPHLGSAVKEVRLEIERQAAMNIIQALAGEKPMGAINQPYPGVKAA"
     gene            3765..4775
                     /gene="OPTptxD"
                     /label=OPTptxD
     regulatory      4776..5513
                     /regulatory_class="terminator"
                     /note="IPCS terminator"
     misc_feature    5514..8596
                     /note="derived from YEp24 yeast extrachromosomal 
                     plasmid(L09156.1)"
     CDS             6153..6344
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    6446..6586
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      6772..7360
     CDS             complement(7531..8391)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="BAP74306.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(7531..8391)
                     /gene="bla"
                     /label=bla
     promoter        complement(8392..8496)
                     /gene="bla"
                     /label=AmpR promoter