LOCUS       Exported               11172 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSCCmec, complete sequence.
ACCESSION   KY701739
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Wu Z.
  TITLE     Functional analyses of CcrC2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11172)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2017) College of Animal Science & Technology, 
            Northwest A&F University, 22 St. Xinong, Yangling, Shaanxi 712100, 
            China
REFERENCE   3  (bases 1 to 11172)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Cloning vector pSCCmec"
                     /mol_type="other DNA"
                     /db_xref="taxon:2022388"
     misc_feature    4..144
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             1313..1912
                     /codon_start=1
                     /product="RepF"
                     /label=RepF
                     /note="temperature-sensitive replicon from pE194"
                     /protein_id="ASN64784.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     CDS             3263..3913
                     /codon_start=1
                     /product="Cat"
                     /label=Cat
                     /protein_id="ASN64785.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     regulatory      4575..4673
                     /regulatory_class="promoter"
                     /note="PmecA"
     CDS             4671..6431
                     /codon_start=1
                     /product="N-terminal tagged CcrC2"
                     /label=N-terminal tagged CcrC2
                     /protein_id="ASN64786.1"
                     /translation="MGFFSKITDKQKHTINKTKKGAGATMKRKVAIYTRVSTKEQLKQG
                     YSIQQQADLLIKKAKQIFPDDEYEIYSDEGISGKNIEARPGMKRLLEDIENRQIKVVMS
                     WKLNRLSRSNRDIQNIIYEFRRGGAYYISISENIDTSTQNGEMMIGAFGLVAQIERETI
                     VSNVKMGMNAKAKQGEAITGRVLGYVLKPNPLTGKNELVIDEFEANIVRQIFDLYLNQN
                     KGLKAIANYLNKQGYHTINKKPFSVYGVKYILNNPVYKGYVRFNNYQNWAVERRSGKND
                     KSKVILVQGKHKAIIDEEIFDKTHEKLAAKSFKPGRPIGGDFWLRGLIKCPECGNNMVC
                     RRTYYNTKKSNERTIKRYYICSLFNRAGSAACHSNAIKAEVVERVVYFHLRRILSQPHV
                     VKTIATQVIDAMEKKKANQTPMSIDTNSLENQKKKLQKRKERLIDLYLDEEIDKETMQN
                     KQDKLNVQLEKIDEQLKQAEVMNKASQDIAIPNYKKIKGQLYVMLYRFTGYMKKANPEA
                     KNKLMHMLIDSIELTTDKQVKLIKYKIDESLLPQSIKKDCGNFFMPKFNFVINVTKKNR
                     IENLSLLPLF"
     repeat_region   4685..4723
                     /label=attSCC
                     /note="attSCC"
     CDS             6928..8934
                     /codon_start=1
                     /gene="mecA"
                     /product="PBP2a"
                     /label=mecA
                     /protein_id="ASN64787.1"
                     /translation="MKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFKQV
                     YKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKIKTN
                     YGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNNVELA
                     NTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTVKKMDE
                     YLRDFAKKFHLTTNETESRNYPLEKATSHLLGYVGPINSEELKQKEYKGYKDDAVIGKK
                     GLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNNM
                     KNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPLLNKFQITT
                     SPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVVNGNIDLKQA
                     IESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKNLDNEILLADS
                     GYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISKENINLLTDGMQ
                     QVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKDNPNMMMAINVKD
                     VQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            6928..8934
                     /gene="mecA"
                     /label=mecA
     promoter        complement(8976..8994)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9001..9017)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      9054..9140
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        9266..9370
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9371..10231
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ASN64788.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10402..10990
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"