LOCUS       Exported                8695 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSCCcat, complete sequence.
ACCESSION   KY701738
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Wu Z.
  TITLE     Single large serine recombinase mediates the horizontal transfer of 
            SCCmec in staphylococci
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8695)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) College of Animal Science & Technology, 
            Northwest A&F University, 22 St. Xinong, Yangling, Shaanxi 712100, 
            China
REFERENCE   3  (bases 1 to 8695)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Cloning vector pSCCcat"
                     /mol_type="other DNA"
                     /db_xref="taxon:2022390"
     misc_feature    4..144
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             1065..1232
                     /codon_start=1
                     /product="Cop-6"
                     /label=Cop-6
                     /protein_id="ASN64779.1"
                     /translation="MVVDRKEEKKVAVTLRLTTEENEILNRIKEKYNISKSDATGILIK
                     KYAKEEYGAF"
     CDS             1313..1912
                     /codon_start=1
                     /product="RepF"
                     /label=RepF
                     /note="TS replicon from pE194"
                     /protein_id="ASN64780.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     CDS             3263..3913
                     /codon_start=1
                     /product="Cat"
                     /label=Cat
                     /protein_id="ASN64781.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     regulatory      4575..4670
                     /regulatory_class="promoter"
                     /note="PmecA"
     CDS             4671..6431
                     /codon_start=1
                     /product="N-terminal-tagged CcrC2"
                     /label=N-terminal-tagged CcrC2
                     /protein_id="ASN64782.1"
                     /translation="MGFFSKITDKQKHTINKTKKGAGATMKRKVAIYTRVSTKEQLKQG
                     YSIQQQADLLIKKAKQIFPDDEYEIYSDEGISGKNIEARPGMKRLLEDIENRQIKVVMS
                     WKLNRLSRSNRDIQNIIYEFRRGGAYYISISENIDTSTQNGEMMIGAFGLVAQIERETI
                     VSNVKMGMNAKAKQGEAITGRVLGYVLKPNPLTGKNELVIDEFEANIVRQIFDLYLNQN
                     KGLKAIANYLNKQGYHTINKKPFSVYGVKYILNNPVYKGYVRFNNYQNWAVERRSGKND
                     KSKVILVQGKHKAIIDEEIFDKTHEKLAAKSFKPGRPIGGDFWLRGLIKCPECGNNMVC
                     RRTYYNTKKSNERTIKRYYICSLFNRAGSAACHSNAIKAEVVERVVYFHLRRILSQPHV
                     VKTIATQVIDAMEKKKANQTPMSIDTNSLENQKKKLQKRKERLIDLYLDEEIDKETMQN
                     KQDKLNVQLEKIDEQLKQAEVMNKASQDIAIPNYKKIKGQLYVMLYRFTGYMKKANPEA
                     KNKLMHMLIDSIELTTDKQVKLIKYKIDESLLPQSIKKDCGNFFMPKFNFVINVTKKNR
                     IENLSLLPLF"
     repeat_region   4685..4723
                     /label=attSCC
                     /note="attSCC"
     promoter        complement(6499..6517)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6524..6540)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      6577..6663
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        6789..6893
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6894..7754
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ASN64783.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7925..8513
                     /direction=RIGHT
                     /label=ori
                     /note="ori"