LOCUS       Exported                4428 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSCANS-2-BNL, complete sequence.
ACCESSION   AF297875
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Dunn JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2000) Biology Department, Brookhaven National 
            Laboratory, 463 Bell Ave., Upton, NY 11973-5000, USA
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Cloning vector pSCANS-2-BNL"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="similar to pSCANS-1-BNL deposited in GenBank 
                     Accession Number AF191089; contains unique recognition 
                     sites SphI and NdeI"
                     /db_xref="taxon:140096"
     CDS             1..816
                     /codon_start=1
                     /gene="kanR"
                     /product="KanR"
                     /label=kanR
                     /note="aminoglycoside 3-phosphotransferase"
                     /protein_id="AAG27437.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            1..816
                     /gene="kanR"
                     /label=kanR
     promoter        961..979
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        984..1002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1097..1115)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1126..1142)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1238..1286)
                     /direction=LEFT
                     /label=f1
                     /note="f1"
     misc_feature    1360..1610
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_binding    complement(1363..1610)
                     /label=IncC binding site
                     /bound_moiety="IncC"
     CDS             complement(1613..2368)
                     /codon_start=1
                     /gene="repE"
                     /product="RepE"
                     /label=repE
                     /protein_id="AAG27438.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(1613..2368)
                     /gene="repE"
                     /label=repE
     rep_origin      2459..2678
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     regulatory      3033..3207
                     /regulatory_class="promoter"
                     /note="contains lac operator sites 1 and 3"
     protein_bind    3098..3119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3134..3164
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3172..3188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3196..3212
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3285..4130
                     /codon_start=1
                     /gene="repL"
                     /product="RepL"
                     /label=repL
                     /protein_id="AAG27439.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCAN
                     LAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANLYR
                     FTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSPCQD
                     DVAIKNKKSQVKKTKRSVSGGAGTTSLKKLTSWIAKAKAKADNLRLSKKRTQKHEFKQK
                     VEAAARKYAYLKNKRSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNEQWGIPAGFRG
                     "
     gene            3285..4130
                     /gene="repL"
                     /label=repL