LOCUS       Exported                8299 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pScaf-5544.2, complete sequence.
ACCESSION   MH319463
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2018) Cellular and Molecular Pharmacology, 
            University of California, San Francisco, 600 16th Street, Genentech 
            Hall S-474, San Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 8299)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Phagemid vector pScaf-5544.2"
                     /mol_type="other DNA"
                     /db_xref="taxon:2268097"
     rep_origin      44..424
                     /note="M13ori; M13 bacteriophage origin of replication; 
                     arrow indicates direction of (+) strand synthesis"
     misc_feature    324..328
                     /label=ori-alpha
                     /note="ori-alpha"
     misc_feature    342..346
                     /label=ori-beta
                     /note="ori-beta"
     misc_feature    351..356
                     /label=ori-gamma
                     /note="ori-gamma"
     misc_feature    360..365
                     /label=ori-delta
                     /note="ori-delta"
     misc_feature    425..3027
                     /label=Insert H
                     /note="Insert H"
     misc_feature    3028..5587
                     /label=Insert C
                     /note="Insert C"
     rep_origin      5588..5917
                     /note="M13term; M13 origin modified with 3-base mutation to
                     eliminate initation capabilities, resulting in M13 
                     terminator"
     misc_feature    5868..5872
                     /label=term-alpha
                     /note="term-alpha"
     misc_feature    5886..5890
                     /label=term-beta
                     /note="term-beta"
     misc_feature    5895..5900
                     /label=term-gamma
                     /note="term-gamma"
     misc_feature    5904..5909
                     /note="term-delta-T3; TGGAAC mutated to TGGTTT"
     primer_bind     complement(5933..5949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5957..5973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5981..6011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6026..6047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6335..6923)
                     /direction=LEFT
                     /note="ori; high-copy number pUC origin of replication"
     CDS             complement(7094..7954)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR; confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics"
                     /protein_id="AXB87504.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(7094..7954)
                     /gene="bla"
                     /label=bla
     regulatory      complement(7955..8059)
                     /regulatory_class="promoter"
                     /note="AmpR promoter"
     promoter        complement(7955..8059)
                     /gene="bla"
                     /label=AmpR promoter