LOCUS       Exported                6689 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSB156, complete sequence.
ACCESSION   EU161577
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Himmelbach A, Zierold U, Hensel G, Riechen J, Douchkov D, Schweizer 
            P, Kumlehn J.
  TITLE     A set of modular binary vectors for transformation of cereals
  JOURNAL   Plant Physiol. 145 (4), 1192-1200 (2007)
  PUBMED    17981986
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Himmelbach A, Zierold U, Hensel G, Riechen J, Douchkov D, Schweizer 
            P, Kumlehn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2007) Cytogenetics and Molecular Cell Biology, 
            Leibniz Institute of Plant Genetics and Crop Plant Research, 
            Corrensstr. 3, Gatersleben D-06466, Germany
REFERENCE   3  (bases 1 to 6689)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Cloning vector pSB156"
                     /mol_type="other DNA"
                     /db_xref="taxon:475873"
     protein_bind    97..118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        133..163
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    171..187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     195..211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        232..250
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    308..1685
                     /label=rice actin (Act-1) promoter and intron 1
                     /note="rice actin (Act-1) promoter and intron 1"
     promoter        317..1149
                     /label=Act1 promoter
                     /note="rice actin promoter"
     CDS             1152..1169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    1735..3738
                     /label=gusINT coding sequence and intron
                     /note="gusINT coding sequence and intron"
     intron          2120..2308
                     /note="PIV2 intron of gusINT; modified potato ST-LS1 
                     intron"
     regulatory      3799..4062
                     /regulatory_class="terminator"
                     /note="nopaline synthase terminator"
     terminator      3809..4061
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      4072..4081
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(4110..4128)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4135..4151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(4292..4747)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4774..4878
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4879..5739
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="ABW70116.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5910..6498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"