LOCUS       Exported                5214 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT4.35S.RNAi, complete sequence.
ACCESSION   EU049859
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Dafny-Yelin M, Chung SM, Frankman EL, Tzfira T.
  TITLE     pSAT RNA interference vectors: a modular series for multiple gene 
            down-regulation in plants
  JOURNAL   Plant Physiol. 145 (4), 1272-1281 (2007)
  PUBMED    17766396
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Dafny-Yelin M, Chung S-M., Frankman EL, Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2007) Department of Molecular, Cellular and 
            Developmental Biology, The University of Michigan, Ann Arbor, MI 
            48109, USA
REFERENCE   3  (bases 1 to 5214)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Cloning vector pSAT4.35S.RNAi"
                     /mol_type="other DNA"
                     /db_xref="taxon:460121"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      429..750
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     regulatory      756..1077
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     promoter        824..1168
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     5UTR           1178..1308
                     /note="translational enhancer; 5 UTR from tobacco etch 
                     virus"
     misc_feature    1309..1349
                     /note="multiple cloning site I; MCS I"
     intron          1350..2702
                     /note="CHSA intron"
     intron          1352..2700
                     /gene="chsA"
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     misc_feature    2703..2746
                     /note="multiple cloning site II; MCS II"
     regulatory      2747..2957
                     /regulatory_class="terminator"
                     /note="CaMV 35S terminator"
     primer_bind     complement(2993..3009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3017..3033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3041..3071)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3086..3107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3395..3983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4154..5014)
                     /codon_start=1
                     /gene="bla"
                     /product="ampicillin resistance protein"
                     /label=bla
                     /note="Amp resistance"
                     /protein_id="ABU39918.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4154..5014)
                     /gene="bla"
                     /label=bla
     promoter        complement(5015..5119)
                     /gene="bla"
                     /label=AmpR promoter