LOCUS       Exported                7095 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Plasmid vector pSAP2-g-multi DNA, complete sequence.
ACCESSION   LC276895
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Miichi F.
  TITLE     Construction of plasmid vector pSAP2-g-multi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Miichi F, Yoshida H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2017) Contact:Fumika Miichi Saga University, 
            Department of Biomolecular Sciences; 5-1-1 Nabeshima, Saga, Saga 
            849-8501, Japan
REFERENCE   3  (bases 1 to 7095)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Plasmid vector pSAP2-g-multi"
                     /mol_type="other DNA"
                     /db_xref="taxon:2015025"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5UTR           659..1249
                     /gene="EhActin 5 region"
     gene            659..1249
                     /gene="EhActin 5 region"
                     /label=EhActin 5 region
     CDS             1250..2044
                     /codon_start=1
                     /gene="neo"
                     /label=neo
                     /protein_id="BBB86758.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            1250..2044
                     /gene="neo"
                     /label=neo
     5UTR           2051..3056
                     /gene="EhActin 3 region"
     gene            2051..3056
                     /gene="EhActin 3 region"
                     /label=EhActin 3 region
     rep_origin      3077..4363
                     /label=Autonomous replication sequence
                     /note="Autonomous replication sequence"
     5UTR           4370..4843
                     /gene="EhAP-a 5 region"
     gene            4370..4843
                     /gene="EhAP-a 5 region"
                     /label=EhAP-a 5 region
     promoter        complement(4910..4928)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4949..4965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4973..4989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4997..5027)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5042..5063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5351..5939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6110..6970)
                     /codon_start=1
                     /gene="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="BBB86759.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(6110..6970)
                     /gene="beta-lactamase"
                     /label=beta-lactamase
     promoter        complement(6971..7075)
                     /gene="bla"
                     /label=AmpR promoter