LOCUS       Exported                5873 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pSal43A, complete sequence.
ACCESSION   LT727506
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5873)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Fungal expression vector pSal43A"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 2360."
                     /db_xref="taxon:1944734"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_feature    651..1735
                     /label=5 flanking region of A. nidulans argB
                     /note="5 flanking region of A. nidulans argB"
     misc_feature    1736..1788
                     /label=5 UTR of A. nidulans argB
                     /note="5 UTR of A. nidulans argB"
     CDS             1789..2868
                     /codon_start=1
                     /note="unnamed protein product; A. nidulans argB"
                     /protein_id="SJL88685.1"
                     /translation="MASLRSVLKSQSLRHTVRSYSSQTMPPASPFAPRHFLSIADLSPS
                     EFATLVRNASSHKRAIKSGSMPQNLQGSLLGKTVAMIFSKRSTRTRVSTEGAVVQMGGH
                     PMFLGKDDIQLGVNESLYDTSVVISSMVSCIVARVGKHAEVADLAKHSSVPVINALCDS
                     FHPLQAVADFQTIYEAFTPKAHHLSSLGLEGLKIAWVGDANNVLFDMAIAATKMGVDIA
                     VATPKGYEIPPHMLELIKSAGEGVSKPGKLLQTNIPEEAVKDADILVTDTWVSMGQEEE
                     KAQRLKEFDGFQITAELAKRGGAKEGWKFMHCLPRHPEEVSDEVFYSNRSLVFPEAENR
                     LWAAISALEGFVVNKGKIE"
     regulatory      2869..3255
                     /regulatory_class="terminator"
                     /note="3 UTR of A. nidulans argB, incl. polyA"
     rep_origin      complement(4028..4264)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(4046..4634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4805..5665)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88686.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5666..5770)
                     /gene="bla"
                     /label=AmpR promoter