LOCUS       Exported                5045 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pSac-Cm, complete sequence.
ACCESSION   AY464562
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Middleton R, Hofmeister A.
  TITLE     New shuttle vectors for ectopic insertion of genes into Bacillus 
            subtilis
  JOURNAL   Plasmid 51 (3), 238-245 (2004)
  PUBMED    15109830
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Middleton RH, Hofmeister AEM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2003) Plant and Microbial Biology, University of 
            California, Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 5045)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Integration vector pSac-Cm"
                     /lab_host="Bacillus subtilis"
                     /mol_type="other DNA"
                     /note="origin of replication and ampicillin resistance for 
                     use in Escherichia coli and integrates into the chromosome 
                     of Bacillus subtilis at the gltA locus; vector pieced 
                     together from the cloning vectors pUC18 and pDG364 and 
                     parts of the sacA gene of Bacillus subtilis"
                     /db_xref="taxon:257529"
     misc_feature    56..700
                     /note="sucrase-6-phosphate hydrolase (sacA) from Bacillus 
                     subtilis"
     CDS             complement(1112..1762)
                     /codon_start=1
                     /gene="CAT"
                     /product="chloramphenicol acetyltransferase"
                     /label=CAT
                     /note="chloramphenicol resistance"
                     /protein_id="AAR30052.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(1112..1762)
                     /gene="CAT"
                     /label=CAT
     misc_feature    2257..2733
                     /note="sucrase-6-phosphate hydrolase (sacA) from Bacillus 
                     subtilis"
     promoter        3139..3243
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3244..4104
                     /codon_start=1
                     /gene="Bla"
                     /product="beta-lactamase"
                     /label=Bla
                     /note="ampicillin resistance"
                     /protein_id="AAR30053.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3244..4104
                     /gene="Bla"
                     /label=Bla
     rep_origin      4275..4863
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"