LOCUS       Exported                7039 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Chloroplast transformation vector pRvdB1, complete sequence.
ACCESSION   DQ073476
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Van Den Bekerom RJM., Hogg B, McCabe MS.
  TITLE     Transformation vector with loxp-sites and aadA for targeting the 
            trnA and trnI region of the tobacco chloroplast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Van Den Bekerom RJM., Hogg B, McCabe MS.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2005) Biology Department, National University of 
            Ireland, Maynooth, Maynooth, County Kildare, Ireland
REFERENCE   3  (bases 1 to 7039)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Chloroplast transformation vector pRvdB1"
                     /lab_host="Nicotiana tabacum"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:332412"
     gene            46..827
                     /pseudo
                     /gene="trnA"
                     /label=trnA
     misc_feature    828..4707
                     /label=similar to PCRIItopo
                     /note="similar to PCRIItopo"
     promoter        complement(847..865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(872..888)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1029..1457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             1801..2595
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2613..3473
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3644..4232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4520..4541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4556..4586
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4594..4610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4618..4634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4652..4670
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     gene            4750..5528
                     /pseudo
                     /gene="trnI"
                     /label=trnI
     misc_feature    5544..5579
                     /label=loxP site
                     /note="loxP site"
     protein_bind    complement(5545..5578)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      5626..5759
                     /regulatory_class="promoter"
                     /note="Prrn promoter in tobacco plastome"
     CDS             5760..6551
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenine transferase"
                     /label=aadA
                     /note="selection marker; confers the resistance to 
                     spectinomycin or streptomycin in bacteria and plants"
                     /protein_id="AAY82481.1"
                     /translation="MAEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            5760..6551
                     /gene="aadA"
                     /label=aadA
     3UTR           6564..6956
                     /label=PsbA-3UTR
                     /note="PsbA-3UTR"
     misc_feature    6998..7033
                     /label=loxP site
                     /note="loxP site"
     protein_bind    complement(6999..7032)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."