LOCUS       Exported               10427 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRV-9, complete sequence.
ACCESSION   AF484679
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Nishizawa M, Fu SL, Kataoka K, Vogt PK.
  TITLE     Artificial oncoproteins: modified versions of the yeast bZip protein
            GCN4 induce cellular transformation
  JOURNAL   Oncogene 22 (39), 7931-7941 (2003)
  PUBMED    12970741
REFERENCE   2  (bases 1 to 10427)
  AUTHORS   Nishizawa M, Fu S-L., Kataoka K, Vogt PK.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2002) Dept. of Molecular and Experimental 
            Medicine, The Scripps Research Institute, 10550 North Torrey Pines 
            Road, La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 10427)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10427
                     /organism="Cloning vector pRV-9"
                     /mol_type="genomic DNA"
                     /note="replication competent; derived from RCAS(BP) avian 
                     retroviral vector developed by Dr. Stephen H. Hughes"
                     /db_xref="taxon:189771"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   412..744
     misc_feature    745..762
                     /note="tRNA-Trp primer binding site for reverse 
                     transcription"
     CDS             1015..3126
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein"
                     /label=gag
                     /note="viral group-specific antigen; precursor protein of 
                     p19, p27, p12 and p15"
                     /protein_id="AAL91561.1"
                     /translation="MEAVIKVISSACKTYCGKISPSKKEIGAMLSLLQKEGLLMSPSDL
                     YSPGSWDPITAALSQRAMVLGKSGELKTWGLVLGALKAAREEQVTSEQAKFWLGLGGGR
                     VSPPGPECIEKPATERRIDKGEEVGETTVQRDAKMAPEEAATPKTVGTSCYHCGTAVGC
                     NCATATASAPPPPYVGSGLYPSLAGVGEQQGQGDNTSRGAEQPREEPGHAGQAPGPALT
                     DWARVREELASTGPPVVAMPVVIKTEGPAWTPLEPKLITRLADTVRTKGLRSPITMAEV
                     EALMSSPLLPHDVTNLMRVILGPAPYALWMDAWGVQLQTVIAAATRDPRHPANGQGRGE
                     RTNLDRLKGLADGMVGNPQGQAALLRPGELVAITASALQAFREVARLAEPAGPWADITQ
                     GPSESFVDFANRLIKAVEGSDLPPSARAPVIIDCFRQKSQPDIQQLIRAAPSTLTTPGE
                     IIKYVLDRQKIAPLTDQGIAAAMSSAIQPLVMAVVNRERDGQTGSGGRARGLCYTCGSP
                     GHYQAQCPKKRKSGNSRERCQLCDGMGHNAKQCRKRDGNQGQRPGRGLSSGPWPGPEQP
                     AVSLAMTMEHKDRPLVRVILTNTGSHPVKQRSVYITALLDSGADITIISEEDWPTDWPV
                     VDTANPQIHGIGGGIPMRKSRDMIEVGVINRDGSLERPLLLFPAVAMVRGSILGRDCLQ
                     GLGLRLTNL"
     gene            1015..3126
                     /gene="gag"
                     /label=gag
     CDS             3144..5831
                     /codon_start=1
                     /gene="pol"
                     /product="reverse trascriptase"
                     /label=pol
                     /note="RNA-dependent DNA polymerase; derived from Bryan 
                     high-titer strain"
                     /protein_id="AAL91562.1"
                     /translation="TVALHLAIPLKWKSDRTPVWIDQWPLPEGKLVALTQLVEKELQLG
                     HIEPSLSCWNTPVFVIRKASGSYRLLHDLRAVNAKLVPFGAVQQGAPVLSALPRGWPLM
                     VLDLKDCFFSIPLAEQDREAFAFTLPSVNNQAPARRFQWKVLPQGMTCSPTICQLVVGQ
                     VLEPLRLKHPALRMLHYMDDLLLAASSHDGLEAAGKEVIGTLERAGFTISPDKIQREPG
                     VQYLGYKLGSTYVAPVGLVAEPRIATLWDVQKLVGSLQWLRPALGIPPRLMGPFYEQLR
                     GSDPNEAREWNLDMKMAWREIVQLSTTAALERWDPAQPLEGAVARCEQGAIGVLGQGLS
                     THPRPCLWLFSTQPTKAFTAWLEVLTLLITKLRASAVRTFGKEVDILLLPACFREDLPL
                     PEGILLALRGFAGKIRSSDTPSIFDIARPLHVSLKVRVTDHPVPGPTVFTDASSSTHKG
                     VVVWREGPRWEIKEIVDLGASVQQLEARAVAMALLLWPTTPTNVVTDSAFVAKMLLKMG
                     QEGVPSTAAAFILEDALSQRSAMAAVLHVRSHSEVPGFFTEGNDVADSQATFQAYPLRE
                     AKDLHTALHIGPRALSKACNISMQQAREVVQTCPHCNSAPALEAGVNPRGLGPLQIWQT
                     DFTLEPRMAPRSWLAVTVDTASSAIVVTQHGRVTSVAAQHHWATAIAVLGRPKAIKTDN
                     GSCFTSRSTREWLARWGIAHTTGIPGNSQGQAMVERANRLLKDKIRVLAEGDGFMKRIP
                     TSKQGELLAKAMYALNHFERGENTKTPIQKHWRPTVLTEGPPVKIRIETGEWEKGWNVL
                     VWGRGYAAVKNRDTDKVIWVPSRKVKPDVTQKDEVTKKDEASPLFAGISDWIPWEDEQE
                     GLQGETASNKQERPGEDTLAANES"
     gene            3144..5831
                     /gene="pol"
                     /label=pol
     CDS             5719..7524
                     /codon_start=1
                     /gene="env"
                     /product="envelope protein"
                     /label=env
                     /note="precursor protein of gp85 and gp37, subgroup A"
                     /protein_id="AAL91563.1"
                     /translation="AFLTGYPGKTSKKDSKEKPLATSKKDPEKTPLLPTRVNYILIIGV
                     LVLCEVTGVRADVHLLEQPGNLWITWANRTGQTDFCLSTQSATSPFQTCLIGIPSPISE
                     GDFKGYVSDTNCTTLGTDRLVSSADFTGGPDNSTTLTYRKVSCLLLKLNVSMWDEPPEL
                     QLLGSQSLPNITNIAQISGITGGCVGFRPQGVPWYLGWSRQEATRFLLRHPSFSKSTEP
                     FTVVTADRHNLFMGSEYCGAYGYRFWNMYNCSQVGRQYRCGNARTPRTGLPEIQCTRRG
                     GKWVNQSQEINESEPFSFTVNCTASSLGNASGCCGKAGTILPGKWVDSTQGSFTKPKAL
                     PPAIFLICGDRAWQGIPSRPVGGPCYLGKLTMLAPKHTDILKVLVNSSRTGIRRKRSTS
                     HLDDTCSDEVQLWGPTARIFASILAPGVAAAQALREIERLACWSVKQANLTTSLLGDLL
                     DDVTSIRHAVLQNRAAIDFLLLAHGHGCEDVAGMCCFNLSDQSESIQKKFQLMKEHVNK
                     IGVDSDLIGSWLRGLFGGIGEWAVHLLKGLLLGLVVILLLVVCLPCLLQMLCGNRRKMI
                     NNSISYHTEYKKLQKACGQPESRIV"
     gene            5719..7524
                     /gene="env"
                     /label=env
     misc_feature    7674..7722
                     /label=polylinker sequence
                     /note="polylinker sequence"
     repeat_region   7835..8167
     primer_bind     complement(8206..8222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8230..8246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8254..8284)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8299..8320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8608..9196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9367..10227)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistant selectable marker"
                     /protein_id="AAL91564.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(9367..10227)
                     /gene="bla"
                     /label=bla
     promoter        complement(10228..10332)
                     /gene="bla"
                     /label=AmpR promoter