LOCUS       Exported                8071 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pRT108, complete sequence.
ACCESSION   AY899296
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Thomas R, Chege G, Shephard E, Bourn W, Maclean J, Jaffray A, Tanzer
            F, Steyn L, Berkower C, Jacobs W Jr., Rybicki E, Williamson A-L.
  TITLE     Construction and stability analysis of different recombinant BCG 
            expressing HIV subtype C Pr55 Gag that induce an immune response in 
            baboons which can be boosted by HIV-1 subtype C Pr55 Gag virus-like 
            particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   Thomas R, Chege G, Shephard E, Bourn W, Maclean J, Jaffray A, Tanzer
            F, Steyn L, Berkower C, Jacobs W Jr., Rybicki E, Williamson A-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2005) Medical Virology, University of Cape Town, 
            Anzio Road, Cape Town, Western Cape 7925, South Africa
REFERENCE   3  (bases 1 to 8071)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Expression vector pRT108"
                     /mol_type="other DNA"
                     /db_xref="taxon:313622"
     CDS             1877..2692
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             6406..7884
                     /codon_start=1
                     /product="Gag"
                     /label=Gag
                     /note="HIV-1 Gag from subtype C isolate DU422 from South 
                     Africa"
                     /protein_id="AAX11267.1"
                     /translation="MGARASILRGEKLDTWEKIRLRPGGKKHYMLKHIVWASRELERFA
                     LNPGLLETSEGCKQIIKQLQPALQTGTEELKSLYDTVATLYCVHEKIEVRDTKEALDKI
                     EEEQNKCQQKTQQAKAADGKVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVIEEKAFSP
                     EVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQMLKDTINEEAAEWDRLHPVHAGPIAP
                     GQMREPRGSDIAGTTSTLQEQIAWMTSNPPIPVGDIYKRWIILGLNKIVRMYSPVSILD
                     IRQGPKEPFRDYVDRFFKTLRAEQATQEVKNWMTDTLLVQNANPDCKTILRALGPGATL
                     EEMMTACQGVGGPGHKARVLAEAMSQTNSGNIMMQRSNFKGPRRIVKCFNCGKEGHIAR
                     NCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQNRPEPTAPPAES
                     FRLEETTPAPKQEPIEREPLTSLKSLFGSDPLSQ"
     terminator      7989..8035
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"