LOCUS       Exported                5026 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pRSVcat, complete sequence.
ACCESSION   DQ075935
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Hastings KEM., Ong J, Zhan S.
  TITLE     pRSVcat chloramphenicol acetyltransferase expression vector 
            (constructed by Gorman et al 1982)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Hastings KEM., Ong J, Zhan S.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2005) Montreal Neurological Institute, McGill 
            University, 3801 University Street, Montreal, QC H3A 2B4, Canada
REFERENCE   3  (bases 1 to 5026)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Expression vector pRSVcat"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:335387"
     repeat_region   1..527
                     /label=RSV LTR
                     /note="RSV LTR"
     promoter        complement(7..268)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_difference 73..74
                     /replace="tta"
                     /compare="J02025.1"
     misc_difference 121
                     /replace="t"
                     /compare="J02025.1"
     misc_difference 248
                     /replace="t"
                     /compare="J02025.1"
     misc_feature    528..5026
                     /label=derived from pSV2cat
                     /note="derived from pSV2cat"
     misc_feature    528..2646
                     /label=derived from pBR322
                     /note="derived from pBR322"
     rep_origin      complement(816..1404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1575..2435)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2436..2540)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    complement(2640..4243)
                     /note="contains SV40 segments for poly(A) addition site and
                     intron sequence"
     polyA_signal    3393..3527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3952..3972)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4102..4167
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(4333..4992)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /protein_id="AAZ03392.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"