LOCUS       Exported                5241 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pRSG43 DNA, complete sequence.
ACCESSION   AB521810
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Yamamoto K, Nishimura M, Kato D, Takeo M, Negoro S.
  TITLE     Identification and characterization of another 4-nitrophenol 
            degradation gene cluster, nps, in Rhodococcus sp. strain PN1
  JOURNAL   J. Biosci. Bioeng. 111 (6), 687-694 (2011)
  PUBMED    21396889
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Takeo M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2009) Contact:Masahiro Takeo University of Hyogo, 
            Graduate School of Engineering, Dept. of Materials Science and 
            Chemistry; 2167 Shosha, Himeji, Hyogo 671-2280, Japan
REFERENCE   3  (bases 1 to 5241)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Shuttle vector pRSG43"
                     /mol_type="other DNA"
                     /note="This Rhodococcus-E.coli shuttle vector pRSG43 was 
                     constructed from Rhodococcus rhodochorus IFO 3338 cryptic 
                     plasmid pRC4 (AB040101) and E.coli vector pHSG299 (M19415).
                     The region from pRC4 (nucleotides 1978-4536 in pRSG43) was 
                     inserted into the AflIII of pHSG299 to construct pRSG43."
                     /db_xref="taxon:674031"
     CDS             327..1142
                     /codon_start=1
                     /gene="agp"
                     /product="aminoglucoside phosphotransferase"
                     /label=agp
                     /protein_id="BAI39642.1"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            327..1142
                     /gene="agp"
                     /label=agp
     CDS             complement(1143..1451)
                     /codon_start=1
                     /gene="lacZ-alpha"
                     /product="modified alpha-polypeptide of beta-galactosidase"
                     /label=lacZ-alpha
                     /protein_id="BAI39643.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF"
     gene            complement(1143..1451)
                     /gene="lacZ-alpha"
                     /label=lacZ-alpha
     primer_bind     1361..1377
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1378..1434
                     /label=multi-cloning site of pHSG299
                     /note="multi-cloning site of pHSG299"
     primer_bind     complement(1447..1463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1471..1487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1495..1525)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1540..1561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             3065..3985
                     /codon_start=1
                     /gene="repA"
                     /product="replicase"
                     /label=repA
                     /protein_id="BAI39644.1"
                     /translation="MDMSGGSLSGDWEQLWLPLWPLATDDLLLGVYRMPRQDALDRRYL
                     EANPQALSNLLVVDVDHPDAALRALSAAGNHPLPNAIVENPRNGHAHAVWALTEPFTRT
                     EYARRKPLAYAAAVNEGLRRAVDGDAAYSGLMTKNPTHSAWDTHWIHAETRSLADLEHD
                     LGKHMPPPRWRQSKRRREDPVGLGRNCMLFETARTWAYRELRCHWGDPEGLGKAIQVEA
                     ADLNAAFSEPLPVSEVRAIAASIHRWIVTKSRMWADGPAVYEATFVAIQSARGRKMTEK
                     KREANRRRATKYDRDLVRKEATDGS"
     gene            3065..3985
                     /gene="repA"
                     /label=repA
     CDS             3975..4256
                     /codon_start=1
                     /gene="repB"
                     /product="DNA-binding replication protein"
                     /label=repB
                     /protein_id="BAI39645.1"
                     /translation="MGAETPARRTRTAREVAERIGASPRTVRRIIAEPRASYEARAAER
                     RKQVLELRASGMKLREIAAEVGMSVGGVGTILHHARKTEQSKAEGAMA"
     gene            3975..4256
                     /gene="repB"
                     /label=repB
     rep_origin      complement(4598..5186)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"