LOCUS       Exported                6333 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRS4110, complete sequence.
ACCESSION   AF174131
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     A new family of yeast vectors and S288C-derived strains for the 
            systematic analysis of gene function
  JOURNAL   Yeast 18 (6), 563-575 (2001)
  PUBMED    11284012
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-1999) Biological Sciences, University of 
            Manchester, 2.205 Stopford Building, Oxford Rd, Manchester M13 9PT, 
            United Kingdom
REFERENCE   3  (bases 1 to 6333)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Cloning vector pRS4110"
                     /mol_type="genomic DNA"
                     /label=yeast centromere vector with ADE8 marker
                     /note="yeast centromere vector with ADE8 marker"
                     /db_xref="taxon:102651"
     misc_feature    332..2230
                     /label=ADE8 gene cassette
                     /note="ADE8 gene cassette"
     CDS             1188..1832
                     /codon_start=1
                     /product="ADE8 protein"
                     /label=ADE8 protein
                     /protein_id="AAD54285.1"
                     /translation="MARIVVLISGSGSNLQALIDAQKQGQLGEDAHIVSVISSSKKAYG
                     LTRAADNNIPTKVCSLYPYTKGIAKEDKAARAKARSQFENDLAKLVLEEKPDVIICAGW
                     LLILGSTFLSQLQSVPILNLHPALPGCFDGTTHAIEMAWRKCQDENKPLTAGCMVHYVI
                     EEVDKGEPLVVKKLEIIPGEETLEQYEQRVHDAEHIAIVEATYKVLQQLHK"
     rep_origin      complement(2790..3245)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(3222..3602)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     3386..3402
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3412..3430
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3439..3546
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     misc_feature    3443..3545
                     /label=polycloning site
                     /note="polycloning site"
     primer_bind     3456..3472
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3506..3522)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3559..3577)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3598..3614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3622..3638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3646..3676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(4000..4588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4759..5619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5620..5724)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5761..6264
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"